Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

C-Type Lectin Domain Family 4, Member M (CLEC4M) (AA 71-399) protein (His tag)

Crystallography grade CLEC4M Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3091840
  • Target Alle C-Type Lectin Domain Family 4, Member M (CLEC4M) Proteine anzeigen
    C-Type Lectin Domain Family 4, Member M (CLEC4M)
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 71-399
    Spezies
    • 8
    • 1
    Human
    Quelle
    • 5
    • 1
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    His tag
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    QVSKVPSSLS QEQSEQDAIY QNLTQLKAAV GELSEKSKLQ EIYQELTQLK AAVGELPEKS KLQEIYQELT RLKAAVGELP EKSKLQEIYQ ELTRLKAAVG ELPEKSKLQE IYQELTRLKA AVGELPEKSK LQEIYQELTE LKAAVGELPE KSKLQEIYQE LTQLKAAVGE LPDQSKQQQI YQELTDLKTA FERLCRHCPK DWTFFQGNCY FMSNSQRNWH DSVTACQEVR AQLVVIKTAE EQNFLQLQTS RSNRFSWMGL SDLNQEGTWQ WVDGSPLSPS FQRYWNSGEP NNSGNEDCAE FSGSGWNDNR CDVDNYWICK KPAACFRDE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CLEC4M Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product CLEC4M Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    C-Type Lectin Domain Family 4, Member M (CLEC4M)
    Andere Bezeichnung
    CLEC4M (CLEC4M Produkte)
    Synonyme
    CD209L Protein, CD299 Protein, DC-SIGN2 Protein, DC-SIGNR Protein, DCSIGNR Protein, HP10347 Protein, L-SIGN Protein, LSIGN Protein, CD209B Protein, C-type lectin domain family 4 member M Protein, CLEC4M Protein
    Hintergrund
    Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. {ECO:0000269|PubMed:11257134}., (Microbial infection) Acts as an attachment receptor for ebola virus (PubMed:12050398, PubMed:12502850). Acts as an attachment receptor for hepatitis C virus (PubMed:15371595, PubMed:16816373). Acts as an attachment receptor for HIV-1 (PubMed:21277928, PubMed:12502850). Acts as an attachment receptor for human coronavirus 229E (PubMed:17037540). Acts as an attachment receptor for human cytomegalovirus/HHV-5 (PubMed:12433371). Acts as an attachment receptor for influenzavirus (PubMed:21191006). Acts as an attachment receptor for SARS coronavirus (PubMed:15479853). Acts as an attachment receptor for west-nile virus (PubMed:16415006). Acts as an attachment receptor for Japanese encephalitis virus (PubMed:24623090). Acts as an attachment receptor for Marburg virus glycoprotein (PubMed:15479853). {ECO:0000269|PubMed:12050398, ECO:0000269|PubMed:12433371, ECO:0000269|PubMed:12502850, ECO:0000269|PubMed:15371595, ECO:0000269|PubMed:15479853, ECO:0000269|PubMed:16415006, ECO:0000269|PubMed:16816373, ECO:0000269|PubMed:17037540, ECO:0000269|PubMed:21191006, ECO:0000269|PubMed:21277928, ECO:0000269|PubMed:24623090}.
    Molekulargewicht
    38.7 kDa Including tag.
    UniProt
    Q9H2X3
Sie sind hier:
Kundenservice