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CLCA2 Protein (AA 32-901) (His tag)

Crystallography grade CLCA2 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3091737
  • Target Alle CLCA2 Proteine anzeigen
    CLCA2 (Chloride Channel Accessory 2 (CLCA2))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 32-901
    Spezies
    • 3
    • 2
    Human
    Quelle
    • 3
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses CLCA2 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    GVQLQDNGYN GLLIAINPQV PENQNLISNI KEMITEASFY LFNATKRRVF FRNIKILIPA TWKANNNSKI KQESYEKANV IVTDWYGAHG DDPYTLQYRG CGKEGKYIHF TPNFLLNDNL TAGYGSRGRV FVHEWAHLRW GVFDEYNNDK PFYINGQNQI KVTRCSSDIT GIFVCEKGPC PQENCIISKL FKEGCTFIYN STQNATASIM FMQSLSSVVE FCNASTHNQE APNLQNQMCS LRSAWDVITD SADFHHSFPM NGTELPPPPT FSLVQAGDKV VCLVLDVSSK MAEADRLLQL QQAAEFYLMQ IVEIHTFVGI ASFDSKGEIR AQLHQINSND DRKLLVSYLP TTVSAKTDIS ICSGLKKGFE VVEKLNGKAY GSVMILVTSG DDKLLGNCLP TVLSSGSTIH SIALGSSAAP NLEELSRLTG GLKFFVPDIS NSNSMIDAFS RISSGTGDIF QQHIQLESTG ENVKPHHQLK NTVTVDNTVG NDTMFLVTWQ ASGPPEIILF DPDGRKYYTN NFITNLTFRT ASLWIPGTAK PGHWTYTLNN THHSLQALKV TVTSRASNSA VPPATVEAFV ERDSLHFPHP VMIYANVKQG FYPILNATVT ATVEPETGDP VTLRLLDDGA GADVIKNDGI YSRYFFSFAA NGRYSLKVHV NHSPSISTPA HSIPGSHAMY VPGYTANGNI QMNAPRKSVG RNEEERKWGF SRVSSGGSFS VLGVPAGPHP DVFPPCKIID LEAVKVEEEL TLSWTAPGED FDQGQATSYE IRMSKSLQNI QDDFNNAILV NTSKRNPQQA GIREIFTFSP QISTNGPEHQ PNGETHESHR IYVAIRAMDR NSLQSAVSNI AQAPLFIPPN SDPVPARDYL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CLCA2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product CLCA2 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    CLCA2 (Chloride Channel Accessory 2 (CLCA2))
    Andere Bezeichnung
    CLCA2 (CLCA2 Produkte)
    Synonyme
    CACC Protein, CACC3 Protein, CLCRG2 Protein, CaCC-3 Protein, CLCA Protein, Clca4 Protein, rbCLCA Protein, 4732440A06 Protein, AI586120 Protein, Clca2 Protein, chloride channel accessory 2 Protein, calcium-activated chloride channel regulator 2 Protein, chloride channel calcium activated 2 Protein, chloride channel calcium activated 5 Protein, CLCA2 Protein, LOC100081268 Protein, Clca2 Protein, Clca5 Protein
    Hintergrund
    Plays a role in modulating chloride current across the plasma membrane in a calcium-dependent manner, and cell adhesion. Involved in basal cell adhesion and/or stratification of squamous epithelia. May act as a tumor suppressor in breast and colorectal cancer. Plays a key role for cell adhesion in the beginning stages of lung metastasis via the binding to ITGB4. {ECO:0000269|PubMed:10554024, ECO:0000269|PubMed:11320086, ECO:0000269|PubMed:11445004, ECO:0000269|PubMed:15707651, ECO:0000269|PubMed:16158324}.
    Molekulargewicht
    97.0 kDa Including tag.
    UniProt
    Q9UQC9
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