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CD79a Protein (AA 33-226) (rho-1D4 tag)

Crystallography grade CD79A Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Produktnummer ABIN3091321
  • Target Alle CD79a (CD79A) Proteine anzeigen
    CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 33-226
    Spezies
    • 9
    • 4
    • 1
    • 1
    • 1
    Human
    Quelle
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses CD79a Protein ist gelabelt mit rho-1D4 tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequenz
    LWMHKVPASL MVSLGEDAHF QCPHNSSNNA NVTWWRVLHG NYTWPPEFLG PGEDPNGTLI IQNVNKSHGG IYVCRVQEGN ESYQQSCGTY LRVRQPPPRP FLDMGEGTKN RIITAEGIIL LFCAVVPGTL LLFRKRWQNE KLGLDAGDEY EDENLYEGLN LDDCSMYEDI SRGLQGTYQD VGSLNIGDVQ LEKP
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CD79A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin-free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product CD79A Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))
    Andere Bezeichnung
    CD79A (CD79A Produkte)
    Synonyme
    CD79A Protein, Ig-alpha Protein, Iga Protein, Igalpha Protein, Ly-54 Protein, Ly54 Protein, mb-1 Protein, IGA Protein, MB-1 Protein, IG-alpha Protein, CD79a molecule Protein, CD79A antigen (immunoglobulin-associated alpha) Protein, Cd79a molecule-like Protein, CD79A Protein, Cd79a Protein, Cd79al Protein
    Hintergrund
    Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells. {ECO:0000269|PubMed:8617796, ECO:0000269|PubMed:9057631}.
    Molekulargewicht
    23.0 kDa Including tag.
    UniProt
    P11912
    Pathways
    BCR Signaling
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