CDC23 Protein (AA 2-597) (His tag)

Produktnummer ABIN3091128

Produktdetails

Target: CDC23 (Cell Division Cycle 23 (CDC23))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-597

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses CDC23 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: AASTSMVPVA VTAAVAPVLS INSDFSDLRE IKKQLLLIAG LTRERGLLHS SKWSAELAFS LPALPLAELQ PPPPITEEDA QDMDAYTLAK AYFDVKEYDR AAHFLHGCNS KKAYFLYMYS RYLSGEKKKD DETVDSLGPL EKGQVKNEAL RELRVELSKK HQARELDGFG LYLYGVVLRK LDLVKEAIDV FVEATHVLPL HWGAWLELCN LITDKEMLKF LSLPDTWMKE FFLAHIYTEL QLIEEALQKY QNLIDVGFSK SSYIVSQIAV AYHNIRDIDK ALSIFNELRK QDPYRIENMD TFSNLLYVRS MKSELSYLAH NLCEIDKYRV ETCCVIGNYY SLRSQHEKAA LYFQRALKLN PRYLGAWTLM GHEYMEMKNT SAAIQAYRHA IEVNKRDYRA WYGLGQTYEI LKMPFYCLYY YRRAHQLRPN DSRMLVALGE CYEKLNQLVE AKKCYWRAYA VGDVEKMALV KLAKLHEQLT ESEQAAQCYI KYIQDIYSCG EIVEHLEEST AFRYLAQYYF KCKLWDEAST CAQKCCAFND TREEGKALLR QILQLRNQGE TPTTEVPAPF FLPASLSANN TPTRRVSPLN LSSVTP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CDC23 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CDC23 (Cell Division Cycle 23 (CDC23))

Andere Bezeichnung: CDC23 (CDC23 Produkte)

Synonyme: CDC23 Protein, anaphase-promoting complex subunit 8 Protein, zgc:56013 Protein, 6030435O18 Protein, D18Ertd243e Protein, ANAPC8 Protein, APC8 Protein, CUT23 Protein, cell division cycle 23 Protein, cell division cycle protein 23 homolog Protein, anaphase-promoting complex subunit 8 Protein, CDC23 (cell division cycle 23, yeast, homolog) Protein, cell division cycle 23 S homeolog Protein, CDC23 cell division cycle 23 Protein, CDC23 Protein, LOC100162672 Protein, LOC100380698 Protein, APC8 Protein, cdc23 Protein, cdc23.S Protein, Cdc23 Protein

Hintergrund: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. {ECO:0000269|PubMed:18485873}.

Molekulargewicht: 69.7 kDa Including tag.

UniProt: Q9UJX2

Pathways: Protein targeting to Nucleus