CDH18 Protein (AA 54-608) (MBP tag)

Produktnummer ABIN3090475

Produktdetails

Target: CDH18 (Cadherin 18, Type 2 (CDH18))

Protein-Typ: Recombinant

Proteineigenschaft: AA 54-608

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses CDH18 Protein ist gelabelt mit MBP tag.

Applikation: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: GWVWNQFFVL EEHMGPDPQY VGKLHSNSDK GDGSVKYILT GEGAGTIFII DDTTGDIHST KSLDREQKTH YVLHAQAIDR RTNKPLEPES EFIIKVQDIN DNAPKFTDGP YIVTVPEMSD MGTSVLQVTA TDADDPTYGN SARVVYSILQ GQPYFSVDPK TGVIRTALHN MDREAREHYS VVIQAKDMAG QVGGLSGSTT VNITLTDVND NPPRFPQKHY QLYVPESAQV GSAVGKIKAN DADTGSNADM TYSIINGDGM GIFSISTDKE TREGILSLKK PLNYEKKKSY TLNIEGANTH LDFRFSHLGP FKDATMLKII VGDVDEPPLF SMPSYLMEVY ENAKIGTVVG TVLAQDPDST NSLVRYFINY NVEDDRFFNI DANTGTIRTT KVLDREETPW YNITVTASEI DNPDLLSHVT VGIRVLDVND NPPELAREYD IIVCENSKPG QVIHTISATD KDDFANGPRF NFFLDERLPV NPNFTLKDNE DNTASILTRR RRFSRTVQDV YYLPIMISDG GIPSLSSSST LTIRVCACER DGRVRTCHAE AFLSS
Sequence without tag.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CDH18 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: The protein is purified from the cleared cell lysate using MBP capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

N-terminal MBP-Tag

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: in solution (20 mM Hepes, pH 7.4, 5 mM NaCl)

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CDH18 (Cadherin 18, Type 2 (CDH18))

Andere Bezeichnung: CDH18 (CDH18 Produkte)

Synonyme: B230220E17Rik Protein, Cdh14l Protein, CDH14 Protein, CDH14L Protein, CDH24 Protein, cadherin 18 Protein, Cdh18 Protein, CDH18 Protein

Hintergrund: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.

Molekulargewicht: 62.6 kDa Including tag.

UniProt: Q13634

Pathways: Cell-Cell Junction Organization