Calcium Channel, Voltage Dependent, T Type, alpha 1I Subunit (CACNA1I) (AA 233-377) protein (His tag)

Details zu Produkt Nr. ABIN3090123, Anbieter: Anmelden zum Anzeigen
Proteinname
  • CACNA1L
  • Ca(V)3.3
  • Cav3.3
  • ca(v)3.3
  • si:ch211-51h13.1
  • Ca(v)3.3
  • calcium voltage-gated channel subunit alpha1 I
  • voltage-dependent T-type calcium channel subunit alpha-1I
  • calcium channel, voltage-dependent, alpha 1I subunit
  • calcium channel, voltage-dependent, T type, alpha 1I subunit
  • CACNA1I
  • LOC100555412
  • Cacna1i
  • cacna1i
Proteineigenschaft
AA 233-377
1
1
1
1
1
Spezies
Human
4
Quelle
Insektenzellen
3
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
Dieses CACNA1I Protein ist gelabelt mit His tag.
Applikation
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Sequenz GLLRNRCFLE ENFTIQGDVA LPPYYQPEED DEMPFICSLS GDNGIMGCHE IPPLKEQGRE CCLSKDDVYD FGAGRQDLNA SGLCVNWNRY YNVCRTGSAN PHKGAINFDN IGYAWIVIFQ VITLEGWVEI MYYVMDAHSF YNFIY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Produktmerkmale
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human CACNA1I Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

Reinigung Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Reinheit >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Protein is endotoxin free.
Güteklasse Crystallography grade
Hintergrund Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. This channel gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes. Gates in voltage ranges similar to, but higher than alpha 1G or alpha 1H (By similarity). {ECO:0000250}.
Molekulargewicht 17.6 kDa Including tag.
UniProt Q9P0X4
Applikations-hinweise In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Kommentare

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C.
Haltbarkeit Unlimited (if stored properly)
Bilder des Herstellers
 image for Calcium Channel, Voltage Dependent, T Type, alpha 1I Subunit (CACNA1I) (AA 233-377) protein (His tag) (ABIN3090123) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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