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MTHFD1 Protein (AA 2-935) (His tag)

Crystallography grade MTHFD1 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3090099
  • Target Alle MTHFD1 Proteine anzeigen
    MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-935
    Spezies
    • 3
    • 2
    Human
    Quelle
    • 3
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses MTHFD1 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    APAEILNGKE ISAQIRARLK NQVTQLKEQV PGFTPRLAIL QVGNRDDSNL YINVKLKAAE EIGIKATHIK LPRTTTESEV MKYITSLNED STVHGFLVQL PLDSENSINT EEVINAIAPE KDVDGLTSIN AGRLARGDLN DCFIPCTPKG CLELIKETGV PIAGRHAVVV GRSKIVGAPM HDLLLWNNAT VTTCHSKTAH LDEEVNKGDI LVVATGQPEM VKGEWIKPGA IVIDCGINYV PDDKKPNGRK VVGDVAYDEA KERASFITPV PGGVGPMTVA MLMQSTVESA KRFLEKFKPG KWMIQYNNLN LKTPVPSDID ISRSCKPKPI GKLAREIGLL SEEVELYGET KAKVLLSALE RLKHRPDGKY VVVTGITPTP LGEGKSTTTI GLVQALGAHL YQNVFACVRQ PSQGPTFGIK GGAAGGGYSQ VIPMEEFNLH LTGDIHAITA ANNLVAAAID ARIFHELTQT DKALFNRLVP SVNGVRRFSD IQIRRLKRLG IEKTDPTTLT DEEINRFARL DIDPETITWQ RVLDTNDRFL RKITIGQAPT EKGHTRTAQF DISVASEIMA VLALTTSLED MRERLGKMVV ASSKKGEPVS AEDLGVSGAL TVLMKDAIKP NLMQTLEGTP VFVHAGPFAN IAHGNSSIIA DRIALKLVGP EGFVVTEAGF GADIGMEKFF NIKCRYSGLC PHVVVLVATV RALKMHGGGP TVTAGLPLPK AYIQENLELV EKGFSNLKKQ IENARMFGIP VVVAVNAFKT DTESELDLIS RLSREHGAFD AVKCTHWAEG GKGALALAQA VQRAAQAPSS FQLLYDLKLP VEDKIRIIAQ KIYGADDIEL LPEAQHKAEV YTKQGFGNLP ICMAKTHLSL SHNPEQKGVP TGFILPIRDI RASVGAGFLY PLVGTMSTMP GLPTRPCFYD IDLDPETEQV NGLF
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human MTHFD1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product MTHFD1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))
    Andere Bezeichnung
    MTHFD1 (MTHFD1 Produkte)
    Synonyme
    MTHFC Protein, MTHFD Protein, Tb07.27M11.970 Protein, Dcs Protein, E430024A07Rik Protein, Mthfd Protein, GB13160 Protein, cb325 Protein, fc29f07 Protein, mthfd1 Protein, wu:fc29f07 Protein, zgc:55597 Protein, methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 Protein, C-1-tetrahydrofolate synthase, cytoplasmic Protein, putative C-1-tetrahydrofolate synthase, cytoplasmic Protein, putative C-1-tetrahydrofolate synthase,cytoplasmic Protein, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like Protein, methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase Protein, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1b Protein, c-1-tetrahydrofolate synthase, cytoplasmic Protein, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase L homeolog Protein, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase Protein, MTHFD1 Protein, Mthfd1 Protein, Tc00.1047053509509.20 Protein, Tc00.1047053511741.50 Protein, Tc00.1047053511517.60 Protein, Tc00.1047053508207.240 Protein, Tb927.7.1600 Protein, LINJ_26_0310 Protein, LBRM_26_0350 Protein, LMJF_26_0320 Protein, mthfd1l Protein, LOC550749 Protein, mthfd1b Protein, Tsp_00651 Protein, mthfd1.L Protein, mthfd1 Protein, LOC589933 Protein, LOC100634307 Protein
    Molekulargewicht
    102.4 kDa Including tag.
    UniProt
    P11586
    Pathways
    Methionine Biosynthetic Process
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