BICD2 Protein (AA 2-824) (His tag)
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- Target Alle BICD2 Produkte
- BICD2 (Bicaudal D Homolog 2 (BICD2))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 2-824
- Spezies
- Human
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses BICD2 Protein ist gelabelt mit His tag.
- Applikation
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequenz
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SAPSEEEEYA RLVMEAQPEW LRAEVKRLSH ELAETTREKI QAAEYGLAVL EEKHQLKLQF EELEVDYEAI RSEMEQLKEA FGQAHTNHKK VAADGESREE SLIQESASKE QYYVRKVLEL QTELKQLRNV LTNTQSENER LASVAQELKE INQNVEIQRG RLRDDIKEYK FREARLLQDY SELEEENISL QKQVSVLRQN QVEFEGLKHE IKRLEEETEY LNSQLEDAIR LKEISERQLE EALETLKTER EQKNSLRKEL SHYMSINDSF YTSHLHVSLD GLKFSDDAAE PNNDAEALVN GFEHGGLAKL PLDNKTSTPK KEGLAPPSPS LVSDLLSELN ISEIQKLKQQ LMQMEREKAG LLATLQDTQK QLEHTRGSLS EQQEKVTRLT ENLSALRRLQ ASKERQTALD NEKDRDSHED GDYYEVDING PEILACKYHV AVAEAGELRE QLKALRSTHE AREAQHAEEK GRYEAEGQAL TEKVSLLEKA SRQDRELLAR LEKELKKVSD VAGETQGSLS VAQDELVTFS EELANLYHHV CMCNNETPNR VMLDYYREGQ GGAGRTSPGG RTSPEARGRR SPILLPKGLL APEAGRADGG TGDSSPSPGS SLPSPLSDPR REPMNIYNLI AIIRDQIKHL QAAVDRTTEL SRQRIASQEL GPAVDKDKEA LMEEILKLKS LLSTKREQIT TLRTVLKANK QTAEVALANL KSKYENEKAM VTETMMKLRN ELKALKEDAA TFSSLRAMFA TRCDEYITQL DEMQRQLAAA EDEKKTLNSL LRMAIQQKLA LTQRLELLEL DHEQTRRGRA KAAPKTKPAT PSL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human BICD2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Kommentare
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- BICD2 (Bicaudal D Homolog 2 (BICD2))
- Andere Bezeichnung
- BICD2 (BICD2 Produkte)
- Synonyme
- SMALED2 Protein, bA526D8.1 Protein, 0610027D24Rik Protein, 1110005D12Rik Protein, AA408834 Protein, mKIAA0699 Protein, MGC84972 Protein, BICD2 Protein, bicd2 Protein, BICD cargo adaptor 2 Protein, bicaudal D homolog 2 (Drosophila) Protein, BICD cargo adaptor 2 L homeolog Protein, zgc:171572 Protein, BICD2 Protein, Bicd2 Protein, bicd2.L Protein, bicd2 Protein, zgc:171572 Protein
- Hintergrund
- May play a role in the dynein-dynactin interactions on the surface of membranous organelles, by associating with these complexes. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex (By similarity). {ECO:0000250}.
- Molekulargewicht
- 94.4 kDa Including tag.
- UniProt
- Q8TD16
- Pathways
- Maintenance of Protein Location
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