ATAD2B Protein (AA 1-1458) (Strep Tag)

Produktnummer ABIN3089547

Produktdetails

Target: ATAD2B (ATPase Family, AAA Domain Containing 2B (ATAD2B))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1458

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses ATAD2B Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MVNTRKSSLR LLGSKSPGPG PGPGAGAEPG ATGGSSHFIS SRTRSSKTRA ASCPAAKAGG SGGAGVTLDE ARKVEVDGSL SDSHVSPPAK RTLKQPDSVC KDKSKSRSTG QREEWNLSTG QARLTSQPGA TLPNGHSGLS LRSHPLRGEK KGDGDLSCIN GDMEVRKSCR SRKNRFESVN QSLLFDQLVN STAEAVLQEM DNINIRRNRR SGEVERLRMW TDTEFENMDM YSRVKRRRKS LRRNSYGIQN HHEVSTEGEE EESQEEDGDI EVEEAEGEEN DRPYNLRQRK TVDRYQAPPI VPAHQKKREN TLFDIHRSPA RRSHIRRKKH AIHSSDTTSS DEERFERRKS KSMARARNRC LPMNFRAEDL ASGILRERVK VGASLADVDP MNIDKSVRFD SIGGLSHHIH ALKEMVVFPL LYPEIFEKFK IQPPRGCLFY GPPGTGKTLV ARALANECSQ GDKKVAFFMR KGADCLSKWV GESERQLRLL FDQAYLMRPS IIFFDEIDGL APVRSSRQDQ IHSSIVSTLL ALMDGLDNRG EIVVIGATNR LDSIDPALRR PGRFDREFLF NLPDQKARKH ILQIHTRDWN PKLSDAFLGE LAEKCVGYCG ADIKALCTEA ALIALRRRYP QIYASSHKLQ LDVSSIVLSA QDFYHAMQNI VPASQRAVMS SGHALSPIIR PLLERSFNNI LAVLQKVFPH AEISQSDKKE DIETLILEDS EDENALSIFE TNCHSGSPKK QSSSAAIHKP YLHFTMSPYH QPTSYRPRLL LSGERGSGQT SHLAPALLHT LERFSVHRLD LPALYSVSAK TPEESCAQIF REARRTVPSI VYMPHIGDWW EAVSETVRAT FLTLLQDIPS FSPIFLLSTS ETMYSELPEE VKCIFRIQYE EVLYIQRPIE EDRRKFFQEL ILNQASMAPP RRKHAALCAM EVLPLALPSP PRQLSESEKS RMEDQEENTL RELRLFLRDV TKRLATDKRF NIFSKPVDIE EVSDYLEVIK EPMDLSTVIT KIDKHNYLTA KDFLKDIDLI CSNALEYNPD KDPGDKIIRH RACTLKDTAH AIIAAELDPE FNKLCEEIKE ARIKRGLSVT SEQINPHSTG ARKTETRVEE AFRHKQRNPM DVWHNSANKC AFRVRRKSRR RSQWGKGIIK KRKVNNLKKD EEDTKFADYE NHTEDRKLLE NGEFEVSTDC HEENGEETGD LSMTNDESSC DIMDLDQGQR LNNGAGTKEN FASTEEESSN ESLLVNSSSS LNPEQTSRKE TFLKGNCLNG EASTDSFEGI PVLECQNGKL EVVSFCDSGD KCSSEQKILL EDQSKEKPET STENHGDDLE KLEALECSNN EKLEPGSDVE VKDAELDKEG ASKVKKYRKL ILEQAKTTSL ELVPEEPSEP VPPLIVDRER LKKLLDLLVD KSNNLAVDQL ERLYSLLSQC IYRHRKDYDK SQLVEEMERT VHMFETFL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ATAD2B (ATPase Family, AAA Domain Containing 2B (ATAD2B))

Andere Bezeichnung: ATAD2B (ATAD2B Produkte)

Synonyme: 1110014E10Rik Protein, BC032887 Protein, C79189 Protein, D530031C13Rik Protein, atad2b Protein, ATPase family, AAA domain containing 2B Protein, ATPase family, AAA domain containing 2B L homeolog Protein, ATAD2B Protein, Atad2b Protein, atad2b.L Protein

Hintergrund: ATPase family AAA domain-containing protein 2B

Molekulargewicht: 164.9 kDa

UniProt: Q9ULI0