ATP1A2 Protein (AA 337-769) (His tag)

Produktnummer ABIN3089526

Produktdetails

Target: ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 337-769

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses ATP1A2 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: TVTVCLTLTA KRMARKNCLV KNLEAVETLG STSTICSDKT GTLTQNRMTV AHMWFDNQIH EADTTEDQSG ATFDKRSPTW TALSRIAGLC NRAVFKAGQE NISVSKRDTA GDASESALLK CIELSCGSVR KMRDRNPKVA EIPFNSTNKY QLSIHEREDS PQSHVLVMKG APERILDRCS TILVQGKEIP LDKEMQDAFQ NAYMELGGLG ERVLGFCQLN LPSGKFPRGF KFDTDELNFP TEKLCFVGLM SMIDPPRAAV PDAVGKCRSA GIKVIMVTGD HPITAKAIAK GVGIISEGNE TVEDIAARLN IPMSQVNPRE AKACVVHGSD LKDMTSEQLD EILKNHTEIV FARTSPQQKL IIVEGCQRQG AIVAVTGDGV NDSPALKKAD IGIAMGISGS DVSKQAADMI LLDDNFASIV TGVEEGRLIF DNL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ATP1A2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))

Andere Bezeichnung: ATP1A2 (ATP1A2 Produkte)

Synonyme: C11orf29 Protein, TIF1D Protein, TIF1DELTA Protein, AW060654 Protein, Atpa-3 Protein, mKIAA0778 Protein, RATATPA2 Protein, FHM2 Protein, MHP2 Protein, atp1a2 Protein, atp[a]2 Protein, atpa2 Protein, cb702 Protein, fa10d04 Protein, fa10d05 Protein, wu:fa10d05 Protein, ATP2A Protein, atp1a2.L Protein, fhm2 Protein, mhp2 Protein, ATP1A2 Protein, ATP1A4 Protein, tripartite motif containing 66 Protein, ATPase, Na+/K+ transporting, alpha 2 polypeptide Protein, ATPase Na+/K+ transporting subunit alpha 2 Protein, ATPase, Na+/K+ transporting, alpha 2a polypeptide Protein, sodium/potassium-transporting ATPase subunit alpha-2 Protein, ATPase, Na+/K+ transporting, alpha 2 polypeptide S homeolog Protein, TRIM66 Protein, Atp1a2 Protein, ATP1A2 Protein, atp1a2a Protein, atp1a2.S Protein, LOC488636 Protein

Hintergrund: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients.

Molekulargewicht: 47.9 kDa Including tag.

UniProt: P50993

Pathways: Thyroid Hormone Synthesis, Proton Transport, Ribonucleoside Biosynthetic Process