ATG12 Protein (AA 1-140) (Strep Tag)

Produktnummer ABIN3089416

Produktdetails

Target: ATG12 (Autophagy Related 12 (ATG12))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-140

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses ATG12 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MAEEPQSVLQ LPTSIAAGGE GLTDVSPETT TPEPPSSAAV SPGTEEPAGD TKKKIDILLK AVGDTPIMKT KKWAVERTRT IQGLIDFIKK FLKLVASEQL FIYVNQSFAP SPDQEVGTLY ECFGSDGKLV LHYCKSQAWG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ATG12 (Autophagy Related 12 (ATG12))

Andere Bezeichnung: ATG12 (ATG12 Produkte)

Synonyme: APG12 Protein, APG12L Protein, FBR93 Protein, HAPG12 Protein, Apg12l Protein, AUTOPHAGY 12 Protein, AUTOPHAGY 12 B Protein, CG10861 Protein, Dmel\\CG10861 Protein, atg12 Protein, ATG12 Protein, apg12 Protein, DDBDRAFT_0185323 Protein, DDBDRAFT_0191412 Protein, DDB_0185323 Protein, DDB_0191412 Protein, 4931423H11Rik Protein, A330058M13Rik Protein, Atg12l Protein, AO090001000593 Protein, autophagy related 12 Protein, Ubiquitin-like superfamily protein Protein, Atg12p Protein, autophagy protein Protein, ubiquitin-like protein ATG12B Protein, autophagy associated protein Atg12 Protein, Autophagy-related 12 Protein, ATG12 autophagy related 12 homolog (S. cerevisiae) Protein, ATG12 autophagy related 12 homolog (S. cerevisiae) pseudogene Protein, autophagy associated ubiquitin-like modifier Atg12 Protein, hypothetical protein Protein, Autophagy-related protein 12 (Autophagy-related ubiquitin-like modifier ATG12) Protein, autophagy protein 12 Protein, autophagy related 12 S homeolog Protein, autophagy-related protein 12 Protein, ATG12 Protein, Atg12 Protein, APG12B Protein, APG12 Protein, LOC9321066 Protein, apg12 Protein, atg12 Protein, LOC743425 Protein, CAALFM_C113950CA Protein, atg12.S Protein, ANI_1_922094 Protein, AOR_1_1064164 Protein

Hintergrund: Ubiquitin-like protein ATG12 (Autophagy-related protein 12) (APG12-like),FUNCTION: Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. {ECO:0000269|PubMed:12207896, ECO:0000269|PubMed:17999726, ECO:0000269|PubMed:19074260, ECO:0000269|PubMed:19164948, ECO:0000269|PubMed:23202584}., FUNCTION: (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established (PubMed:19666601). {ECO:0000269|PubMed:17709747, ECO:0000269|PubMed:19666601}.

Molekulargewicht: 15.1 kDa

UniProt: O94817

Pathways: Autophagie