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ANAPC10 Protein (AA 2-185) (His tag)

Crystallography grade ANAPC10 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3089092
  • Target Alle ANAPC10 Proteine anzeigen
    ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-185
    Spezies
    • 2
    • 1
    • 1
    • 1
    Human
    Quelle
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses ANAPC10 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    TTPNKTPPGA DPKQLERTGT VREIGSQAVW SLSSCKPGFG VDQLRDDNLE TYWQSDGSQP HLVNIQFRRK TTVKTLCIYA DYKSDESYTP SKISVRVGNN FHNLQEIRQL ELVEPSGWIH VPLTDNHKKP TRTFMIQIAV LANHQNGRDT HMRQIKIYTP VEESSIGKFP RCTTIDFMMY RSIR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ANAPC10 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product ANAPC10 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))
    Andere Bezeichnung
    ANAPC10 (ANAPC10 Produkte)
    Synonyme
    APC10 Protein, DOC1 Protein, 1500026N15Rik Protein, A830003M23Rik Protein, CG11419 Protein, Dmel\\CG11419 Protein, ANAPC10 Protein, SPBC1E8.06 Protein, anapc10 Protein, MGC85037 Protein, DDBDRAFT_0202332 Protein, DDBDRAFT_0235160 Protein, DDB_0202332 Protein, DDB_0235160 Protein, anaphase promoting complex subunit 10 Protein, Anaphase Promoting Complex subunit 10 Protein, anaphase promoting complex subunit DOC1 Protein, anaphase-promoting complex subunit Apc10 Protein, anaphase promoting complex subunit Protein, predicted protein Protein, anaphase promoting complex subunit 10 S homeolog Protein, ANAPC10 Protein, Anapc10 Protein, APC10 Protein, DOC1 Protein, apc10 Protein, CAALFM_C107850CA Protein, anapc10 Protein, LOC692856 Protein, anapc10.S Protein, NAEGRDRAFT_66759 Protein
    Hintergrund
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. {ECO:0000269|PubMed:18485873}.
    Molekulargewicht
    22.1 kDa Including tag.
    UniProt
    Q9UM13
    Pathways
    M Phase
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