PRMT1 Protein (AA 1-361) (His tag)

Produktnummer ABIN3088967

Produktdetails

Target: PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-361

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses PRMT1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)

Sequenz: MENFVATLAN GMSLQPPLEE VSCGQAESSE KPNAEDMTSK DYYFDSYAHF GIHEEMLKDE VRTLTYRNSM FHNRHLFKDK VVLDVGSGTG ILCMFAAKAG ARKVIGIECS SISDYAVKIV KANKLDHVVT IIKGKVEEVE LPVEKVDIII SEWMGYCLFY ESMLNTVLYA RDKWLAPDGL IFPDRATLYV TAIEDRQYKD YKIHWWENVY GFDMSCIKDV AIKEPLVDVV DPKQLVTNAC LIKEVDIYTV KVEDLTFTSP FCLQVKRNDY VHALVAYFNI EFTRCHKRTG FSTSPESPYT HWKQTVFYME DYLTVKTGEE IFGTIGMRPN AKNNRDLDFT IDLDFKGQLC ELSCSTDYRM R
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PRMT1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))

Andere Bezeichnung: PRMT1 (PRMT1 Produkte)

Synonyme: ANM1 Protein, HCP1 Protein, HRMT1L2 Protein, IR1B4 Protein, 6720434D09Rik Protein, AW214366 Protein, Hrmt1l2 Protein, Mrmt1 Protein, 3E10 Protein, anm1 Protein, hcp1 Protein, hrmt1l2 Protein, ir1b4 Protein, prmt1 Protein, prmt1b Protein, xPRMT1b Protein, xprmt1 Protein, xPRMT1 Protein, ARABIDOPSIS THALIANA PROTEIN ARGININE METHYLTRANSFERASE 1A Protein, ATPRMT1A Protein, F6F22.30 Protein, F6F22_30 Protein, protein arginine methyltransferase 1A Protein, DDBDRAFT_0183976 Protein, DDBDRAFT_0235399 Protein, DDB_0183976 Protein, DDB_0235399 Protein, PRMT1 Protein, DKFZp459J1326 Protein, fb39h07 Protein, wu:fb39h07 Protein, zf1 Protein, zgc:66201 Protein, protein arginine methyltransferase 1 Protein, protein arginine N-methyltransferase 1 Protein, protein arginine methyltransferase 1 L homeolog Protein, protein arginine methyltransferase 1 S homeolog Protein, protein arginine methyltransferase 1A Protein, protein arginine N-methyltransferase-1 Protein, protein arginine N-methyltransferase Protein, protein arginine methyltransferase Protein, PRMT1 Protein, Prmt1 Protein, prmt1.L Protein, prmt1.S Protein, PRMT1A Protein, prm-1 Protein, EDI_007800 Protein, Smp_029240.3 Protein, prmt1 Protein

Hintergrund: Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789). {ECO:0000269|PubMed:11387442, ECO:0000269|PubMed:11448779, ECO:0000269|PubMed:12718890, ECO:0000269|PubMed:18320585, ECO:0000269|PubMed:18657504, ECO:0000269|PubMed:18773938, ECO:0000269|PubMed:19124016, ECO:0000269|PubMed:19136629, ECO:0000269|PubMed:19405910, ECO:0000269|PubMed:20442406, ECO:0000269|PubMed:25284789}.

Molekulargewicht: 42.5 kDa Including tag.

UniProt: Q99873