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LPHN3 Protein (AA 20-866) (His tag)

Crystallography grade LPHN3 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Produktnummer ABIN3088926
  • Target Alle LPHN3 Proteine anzeigen
    LPHN3 (Latrophilin 3 (LPHN3))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 20-866
    Spezies
    • 3
    • 3
    Human
    Quelle
    • 5
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses LPHN3 Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
    Sequenz
    FSRAPIPMAV VRRELSCESY PIELRCPGTD VIMIESANYG RTDDKICDSD PAQMENIRCY LPDAYKIMSQ RCNNRTQCAV VAGPDVFPDP CPGTYKYLEV QYECVPYKVE QKVFLCPGLL KGVYQSEHLF ESDHQSGAWC KDPLQASDKI YYMPWTPYRT DTLTEYSSKD DFIAGRPTTT YKLPHRVDGT GFVVYDGALF FNKERTRNIV KFDLRTRIKS GEAIIANANY HDTSPYRWGG KSDIDLAVDE NGLWVIYATE QNNGKIVISQ LNPYTLRIEG TWDTAYDKRS ASNAFMICGI LYVVKSVYED DDNEATGNKI DYIYNTDQSK DSLVDVPFPN SYQYIAAVDY NPRDNLLYVW NNYHVVKYSL DFGPLDSRSG QAHHGQVSYI SPPIHLDSEL ERPSVKDIST TGPLGMGSTT TSTTLRTTTL SPGRSTTPSV SGRRNRSTST PSPAVEVLDD MTTHLPSASS QIPALEESCE AVEAREIMWF KTRQGQIAKQ PCPAGTIGVS TYLCLAPDGI WDPQGPDLSN CSSPWVNHIT QKLKSGETAA NIARELAEQT RNHLNAGDIT YSVRAMDQLV GLLDVQLRNL TPGGKDSAAR SLNKAMVETV NNLLQPQALN AWRDLTTSDQ LRAATMLLHT VEESAFVLAD NLLKTDIVRE NTDNIKLEVA RLSTEGNLED LKFPENMGHG STIQLSANTL KQNGRNGEIR VAFVLYNNLG PYLSTENASM KLGTEALSTN HSVIVNSPVI TAAINKEFSN KVYLADPVVF TVKHIKQSEE NFNPNCSFWS YSKRTMTGYW STQGCRLLTT NKTHTTCSCN HLTNFAVLMA HVEVKHSDAV HDLLLDV
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ADGRL3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product LPHN3 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    LPHN3 (Latrophilin 3 (LPHN3))
    Andere Bezeichnung
    ADGRL3 (LPHN3 Produkte)
    Synonyme
    LPHN3 Protein, CIRL3 Protein, LEC3 Protein, 5430402I23Rik Protein, CIRL-3 Protein, D130075K09Rik Protein, Gm1379 Protein, mKIAA0768 Protein, Cirl3 Protein, Lec3 Protein, LPHH3 Protein, adhesion G protein-coupled receptor L3 Protein, ADGRL3 Protein, LOC100554073 Protein, Adgrl3 Protein
    Hintergrund
    Plays a role in cell-cell adhesion and neuron guidance via its interactions with FLRT2 and FLRT3 that are expressed at the surface of adjacent cells (PubMed:26235030). Plays a role in the development of glutamatergic synapses in the cortex. Important in determining the connectivity rates between the principal neurons in the cortex. {ECO:0000250|UniProtKB:Q80TS3, ECO:0000305|PubMed:26235030}.
    Molekulargewicht
    95.4 kDa Including tag.
    UniProt
    Q9HAR2
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