ADPGK Protein (AA 23-497) (His tag)

Produktnummer ABIN3088835

Produktdetails

Target: ADPGK (ADP-Dependent Glucokinase (ADPGK))

Protein-Typ: Recombinant

Proteineigenschaft: AA 23-497

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses ADPGK Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: LEPELPGSAL RSLWSSLCLG PAPAPPGPVS PEGRLAAAWD ALIVRPVRRW RRVAVGVNAC VDVVLSGVKL LQALGLSPGN GKDHSILHSR NDLEEAFIHF MGKGAAAERF FSDKETFHDI AQVASEFPGA QHYVGGNAAL IGQKFAANSD LKVLLCGPVG PKLHELLDDN VFVPPESLQE VDEFHLILEY QAGEEWGQLK APHANRFIFS HDLSNGAMNM LEVFVSSLEE FQPDLVVLSG LHMMEGQSKE LQRKRLLEVV TSISDIPTGI PVHLELASMT NRELMSSIVH QQVFPAVTSL GLNEQELLFL TQSASGPHSS LSSWNGVPDV GMVSDILFWI LKEHGRSKSR ASDLTRIHFH TLVYHILATV DGHWANQLAA VAAGARVAGT QACATETIDT SRVSLRAPQE FMTSHSEAGS RIVLNPNKPV VEWHREGISF HFTPVLVCKD PIRTVGLGDA ISAEGLFYSE VHPHY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ADPGK Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ADPGK (ADP-Dependent Glucokinase (ADPGK))

Andere Bezeichnung: ADPGK (ADPGK Produkte)

Synonyme: ADPGK Protein, 2610017G09Rik Protein, ADP-GK Protein, Adp-gk Protein, C77577 Protein, RGD1306103 Protein, ADP dependent glucokinase Protein, ADP-dependent glucokinase Protein, ADPGK Protein, PH_RS02770 Protein, PF_RS01590 Protein, MA_RS18600 Protein, MM_RS02530 Protein, PAB_RS07820 Protein, MBAR_RS10650 Protein, Mthe_1214 Protein, TON_RS01305 Protein, TGAM_RS04175 Protein, TSIB_RS01230 Protein, PNA2_RS06110 Protein, Adpgk Protein

Hintergrund: Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity). {ECO:0000250}.

Molekulargewicht: 52.7 kDa Including tag.

UniProt: Q9BRR6