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AMIGO1 Protein (AA 28-372) (His tag)

Crystallography grade AMIGO1 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Produktnummer ABIN3088799
  • Target Alle AMIGO1 Proteine anzeigen
    AMIGO1 (Adhesion Molecule with Ig-Like Domain 1 (AMIGO1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 28-372
    Spezies
    • 2
    • 2
    • 1
    Human
    Quelle
    • 3
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses AMIGO1 Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequenz
    GRAVVSCPAA CLCASNILSC SKQQLPNVPH SLPSYTALLD LSHNNLSRLR AEWTPTRLTQ LHSLLLSHNH LNFISSEAFS PVPNLRYLDL SSNQLRTLDE FLFSDLQVLE VLLLYNNHIM AVDRCAFDDM AQLQKLYLSQ NQISRFPLEL VKEGAKLPKL TLLDLSSNKL KNLPLPDLQK LPAWIKNGLY LHNNPLNCDC ELYQLFSHWQ YRQLSSVMDF QEDLYCMNSK KLHNVFNLSF LNCGEYKERA WEAHLGDTLI IKCDTKQQGM TKVWVTPSNE RVLDEVTNGT VSVSKDGSLL FQQVQVEDGG VYTCYAMGET FNETLSVELK VHNFTLHGHH DTLNT
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human AMIGO1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    AMIGO1 (Adhesion Molecule with Ig-Like Domain 1 (AMIGO1))
    Andere Bezeichnung
    AMIGO1 (AMIGO1 Produkte)
    Synonyme
    A Protein, Amigo Protein, ali2 Protein, AMIGO Protein, AMIGO-1 Protein, adhesion molecule with Ig like domain 1 Protein, Amigo1 Protein, AMIGO1 Protein
    Hintergrund
    Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. {ECO:0000250|UniProtKB:Q80ZD7, ECO:0000250|UniProtKB:Q80ZD8}.
    Molekulargewicht
    40.2 kDa Including tag.
    UniProt
    Q86WK6
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