ADH5 Protein (AA 2-374) (His tag)
-
- Target Alle ADH5 Proteine anzeigen
- ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 2-374
-
Spezies
- Human
-
Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses ADH5 Protein ist gelabelt mit His tag.
- Applikation
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- Sequenz
-
ANEVIKCKAA VAWEAGKPLS IEEIEVAPPK AHEVRIKIIA TAVCHTDAYT LSGADPEGCF PVILGHEGAG IVESVGEGVT KLKAGDTVIP LYIPQCGECK FCLNPKTNLC QKIRVTQGKG LMPDGTSRFT CKGKTILHYM GTSTFSEYTV VADISVAKID PLAPLDKVCL LGCGISTGYG AAVNTAKLEP GSVCAVFGLG GVGLAVIMGC KVAGASRIIG VDINKDKFAR AKEFGATECI NPQDFSKPIQ EVLIEMTDGG VDYSFECIGN VKVMRAALEA CHKGWGVSVV VGVAASGEEI ATRPFQLVTG RTWKGTAFGG WKSVESVPKL VSEYMSKKIK VDEFVTHNLS FDEINKAFEL MHSGKSIRTV VKI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Human ADH5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
-
Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product ADH5 Protein
-
-
- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
-
In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
-
- Target
- ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))
- Andere Bezeichnung
- ADH5 (ADH5 Produkte)
- Synonyme
- ADH2 Protein, ADH-3 Protein, ADHX Protein, FALDH Protein, FDH Protein, GSH-FDH Protein, GSNOR Protein, ADH1C Protein, ADH3 Protein, wu:fb60b11 Protein, Adh-5 Protein, Adh3 Protein, adh-3 Protein, adh3 Protein, adh5 Protein, adhx Protein, fdh Protein, gsnor Protein, ADH4 Protein, ADH5 Protein, alcohol dehydrogenase 1B (class I), beta polypeptide Protein, alcohol dehydrogenase 5 (class III), chi polypeptide Protein, alcohol dehydrogenase 5 Protein, alcohol dehydrogenase 5 (class III), chi polypeptide L homeolog Protein, alcohol dehydrogenase class-3 Protein, ADH1B Protein, ADH5 Protein, adh5 Protein, Adh5 Protein, adh5.L Protein, LOC101112223 Protein
- Hintergrund
- Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.
- Molekulargewicht
- 40.5 kDa Including tag.
- UniProt
- P11766
-