ACSL6 Protein (AA 46-697) (His tag)
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- Target Alle ACSL6 Proteine anzeigen
- ACSL6 (Acyl-CoA Synthetase Long-Chain Family Member 6 (ACSL6))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 46-697
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Spezies
- Human
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses ACSL6 Protein ist gelabelt mit His tag.
- Applikation
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequenz
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HRPKALQPPC NLLMQSEEVE DSGGARRSVI GSGPQLLTHY YDDARTMYQV FRRGLSISGN GPCLGFRKPK QPYQWLSYQE VADRAEFLGS GLLQHNCKAC TDQFIGVFAQ NRPEWIIVEL ACYTYSMVVV PLYDTLGPGA IRYIINTADI STVIVDKPQK AVLLLEHVER KETPGLKLII LMDPFEEALK ERGQKCGVVI KSMQAVEDCG QENHQAPVPP QPDDLSIVCF TSGTTGNPKG AMLTHGNVVA DFSGFLKVTE KVIFPRQDDV LISFLPLAHM FERVIQSVVY CHGGRVGFFQ GDIRLLSDDM KALCPTIFPV VPRLLNRMYD KIFSQANTPL KRWLLEFAAK RKQAEVRSGI IRNDSIWDEL FFNKIQASLG GCVRMIVTGA APASPTVLGF LRAALGCQVY EGYGQTECTA GCTFTTPGDW TSGHVGAPLP CNHIKLVDVE ELNYWACKGE GEICVRGPNV FKGYLKDPDR TKEALDSDGW LHTGDIGKWL PAGTLKIIDR KKHIFKLAQG EYVAPEKIEN IYIRSQPVAQ IYVHGDSLKA FLVGIVVPDP EVMPSWAQKR GIEGTYADLC TNKDLKKAIL EDMVRLGKES GLHSFEQVKA IHIHSDMFSV QNGLLTPTLK AKRPELREYF KKQIEELYSI SM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ACSL6 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product ACSL6 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- ACSL6 (Acyl-CoA Synthetase Long-Chain Family Member 6 (ACSL6))
- Andere Bezeichnung
- ACSL6 (ACSL6 Produkte)
- Synonyme
- ACS2 Protein, FACL6 Protein, LACS 6 Protein, LACS2 Protein, LACS5 Protein, ACSL6 Protein, MGC146229 Protein, Facl6 Protein, Lacsl Protein, A330035H04Rik Protein, AW050338 Protein, LACS Protein, mKIAA0837 Protein, wu:fa04h02 Protein, wu:fc05h07 Protein, acyl-CoA synthetase long chain family member 6 Protein, acyl-CoA synthetase long-chain family member 6 Protein, ACSL6 Protein, acsl6 Protein, Acsl6 Protein, LOAG_15918 Protein, LOC416323 Protein
- Hintergrund
- Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid.
- Molekulargewicht
- 73.7 kDa Including tag.
- UniProt
- Q9UKU0
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