Major Histocompatibility Complex, Class I, A (HLA-A) (AA 25-308) protein (His tag)

Details zu Produkt Nr. ABIN3088064, Anbieter: Anmelden zum Anzeigen
Proteinname
  • HLAA
  • major histocompatibility complex, class I, A
  • HLA-A
Proteineigenschaft
AA 25-308
21
19
1
1
Spezies
Human
42
1
Quelle
Escherichia coli (E. coli)
21
21
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
Dieses HLA-A Protein ist gelabelt mit His tag.
Applikation
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Bilder

Sequenz GSHSMRYFYT SVSRPGRGEP RFIAVGYVDD TQFVRFDSDA ASQRMEPRAP WIEQEGPEYW DQETRNVKAQ SQTDRVDLGT LRGYYNQSED GSHTIQIMYG CDVGPDGRFL RGYRQDAYDG KDYIALNEDL RSWTAADMAA QITKRKWEAA HAAEQQRAYL EGRCVEWLRR YLENGKETLQ RTDPPKTHMT HHPISDHEAT LRCWALGFYP AEITLTWQRD GEDQTQDTEL VETRPAGDGT FQKWAAVVVP SGEEQRYTCH VQHEGLPKPL TLRWELSSQP TIPI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Produktmerkmale
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human HLA-A Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Reinigung Two step purification of proteins expressed in bacterial culture:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Reinheit >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Endotoxin has not been removed. Please contact us if you require endotoxin removal.
Güteklasse Crystallography grade
Hintergrund Involved in the presentation of foreign antigens to the immune system.
Molekulargewicht 33.7 kDa Including tag.
UniProt P13746
Forschungsgebiet Major Histocompatibility Complex (MHC), Immunology
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Applikationshinweise In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Kommentare

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C.
Haltbarkeit Unlimited (if stored properly)
Bilder des Herstellers
 image for Major Histocompatibility Complex, Class I, A (HLA-A) (AA 25-308) protein (His tag) (ABIN3088064) „Crystallography Grade“ protein due to multi-step, protein-specific purification process