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SAE1 Protein (AA 2-346) (His tag)

Crystallography grade SAE1 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Produktnummer ABIN3087826
  • Target Alle SAE1 Proteine anzeigen
    SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-346
    Spezies
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 4
    • 3
    • 2
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses SAE1 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequenz
    VEKEEAGGGI SEEEAAQYDR QIRLWGLEAQ KRLRASRVLL VGLKGLGAEI AKNLILAGVK GLTMLDHEQV TPEDPGAQFL IRTGSVGRNR AEASLERAQN LNPMVDVKVD TEDIEKKPES FFTQFDAVCL TCCSRDVIVK VDQICHKNSI KFFTGDVFGY HGYTFANLGE HEFVEEKTKV AKVSQGVEDG PDTKRAKLDS SETTMVKKKV VFCPVKEALE VDWSSEKAKA ALKRTTSDYF LLQVLLKFRT DKGRDPSSDT YEEDSELLLQ IRNDVLDSLG ISPDLLPEDF VRYCFSEMAP VCAVVGGILA QEIVKALSQR DPPHNNFFFF DGMKGNGIVE CLGPK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SAE1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product SAE1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
    Andere Bezeichnung
    SAE1 (SAE1 Produkte)
    Synonyme
    AOS1 Protein, HSPC140 Protein, SUA1 Protein, UBLE1A Protein, 2400010M20Rik Protein, 2610044L12Rik Protein, AL033372 Protein, AW743391 Protein, D7Ertd177e Protein, Sua1 Protein, Uble1a Protein, Aos1p Protein, Sua1p Protein, aos Protein, sae2a Protein, uble1a Protein, wu:fa28b04 Protein, zgc:86633 Protein, SUMO1 activating enzyme subunit 1 Protein, SUMO1 activating enzyme subunit 1 L homeolog Protein, SAE1 Protein, Sae1 Protein, sae1.L Protein, sae1 Protein
    Hintergrund
    The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. {ECO:0000269|PubMed:10187858, ECO:0000269|PubMed:10217437, ECO:0000269|PubMed:11451954, ECO:0000269|PubMed:11481243, ECO:0000269|PubMed:15660128, ECO:0000269|PubMed:20164921, ECO:0000269|PubMed:9920803}.
    Molekulargewicht
    39.3 kDa Including tag.
    UniProt
    Q9UBE0
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