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SLC35G2 Protein (AA 1-412) (rho-1D4 tag)

Crystallography grade SLC35G2 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Produktnummer ABIN3087698
  • Target Alle SLC35G2 Produkte
    SLC35G2 (Solute Carrier Family 35, Member G2 (SLC35G2))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-412
    Spezies
    • 3
    • 1
    Human
    Quelle
    • 3
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses SLC35G2 Protein ist gelabelt mit rho-1D4 tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
    Sequenz
    MDTSPSRKYP VKKRVKIHPN TVMVKYTSHY PQPGDDGYEE INEGYGNFME ENPKKGLLSE MKKKGRAFFG TMDTLPPPTE DPMINEIGQF QSFAEKNIFQ SRKMWIVLFG SALAHGCVAL ITRLVSDRSK VPSLELIFIR SVFQVLSVLV VCYYQEAPFG PSGYRLRLFF YGVCNVISIT CAYTSFSIVP PSNGTTMWRA TTTVFSAILA FLLVDEKMAY VDMATVVCSI LGVCLVMIPN IVDEDNSLLN AWKEAFGYTM TVMAGLTTAL SMIVYRSIKE KISMWTALFT FGWTGTIWGI STMFILQEPI IPLDGETWSY LIAICVCSTA AFLGVYYALD KFHPALVSTV QHLEIVVAMV LQLLVLHIFP SIYDVFGGVI IMISVFVLAG YKLYWRNLRK QDYQEILDSP IK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SLC35G2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin-free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    SLC35G2 (Solute Carrier Family 35, Member G2 (SLC35G2))
    Andere Bezeichnung
    SLC35G2 (SLC35G2 Produkte)
    Synonyme
    TMEM22 Protein, Gm519 Protein, Tmem22 Protein, im:6912006 Protein, tmem22 Protein, zgc:113637 Protein, zgc:101525 Protein, solute carrier family 35 member G2 Protein, solute carrier family 35, member G2 Protein, solute carrier family 35, member G2b Protein, solute carrier family 35, member G2a Protein, SLC35G2 Protein, Slc35g2 Protein, slc35g2b Protein, slc35g2a Protein
    Hintergrund
    May play a role in cell proliferation. {ECO:0000305|PubMed:19148500}.
    Molekulargewicht
    47.6 kDa Including tag.
    UniProt
    Q8TBE7
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