HNRNPA3 Protein (AA 1-378) (His tag)
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- Target Alle HNRNPA3 Proteine anzeigen
- HNRNPA3 (Heterogeneous Nuclear Ribonucleoprotein A3 (HNRNPA3))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-378
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses HNRNPA3 Protein ist gelabelt mit His tag.
- Applikation
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequenz
- MHHHHHHEVK PPPGRPQPDS GRRRRRRGEE GHDPKEPEQL RKLFIGGLSF ETTDDSLREH FEKWGTLTDC VVMRDPQTKR SRGFGFVTYS CVEEVDAAMC ARPHKVDGRV VEPKRAVSRE DSVKPGAHLT VKKIFVGGIK EDTEEYNLRD YFEKYGKIET IEVMEDRQSG KKRGFAFVTF DDHDTVDKIV VQKYHTINGH NCEVKKALSK QEMQSAGSQR GRGGGSGNFM GRGGNFGGGG GNFGRGGNFG GRGGYGGGGG GSRGSYGGGD GGYNGFGGDG GNYGGGPGYS SRGGYGGGGP GYGNQGGGYG GGGGYDGYNE GGNFGGGNYG GGGNYNDFGN YSGQQQSNYG PMKGGSFGGR SSGSPYGGGY GSGGGSGGYG SRRF
- Spezifität
- N-terminal His-tag
- Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human HNRNPA3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount..
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Güteklasse
- Crystallography grade
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 50 mM Tris, pH 7.5; 150 mM NaCl; 2 mM DTT; 2mM EDTA
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- HNRNPA3 (Heterogeneous Nuclear Ribonucleoprotein A3 (HNRNPA3))
- Andere Bezeichnung
- HNRNPA3 (HNRNPA3 Produkte)
- Synonyme
- 2610510D13Rik Protein, D10S102 Protein, FBRNP Protein, HNRPA3 Protein, 2410013L13Rik Protein, 2610209F03Rik Protein, Hnrpa3 Protein, zgc:153703 Protein, DKFZp469I0118 Protein, heterogeneous nuclear ribonucleoprotein A3 Protein, heterogeneous nuclear ribonucleoprotein A3 S homeolog Protein, heterogeneous nuclear ribonucleoprotein A1 Protein, hypothetical protein Protein, HNRNPA3 Protein, Hnrnpa3 Protein, hnrnpa3.S Protein, HNRNPA1 Protein, hnrnpa3 Protein, ARCVE_RS10260 Protein
- Hintergrund
- Plays a role in cytoplasmic trafficking of RNA. Binds to the cis-acting response element, A2RE. May be involved in pre-mRNA splicing. {ECO:0000269|PubMed:11886857}.
- Molekulargewicht
- 40.5 kDa Including tag.
- UniProt
- P51991
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