RGP1 Protein (AA 1-391) (Strep Tag)

Produktnummer ABIN3087216

Produktdetails

Target: RGP1 (RGP1 Retrograde Golgi Transport Homolog (RGP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-391

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses RGP1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MIEVVAELSR GPVFLAGEAL ECVVTVTNPL PPTATSASSE ALAWASAQIH CQFHASESRV ALPPPDSSQP DVQPDSQTVF LPHRGERGQC ILSTPPKILF CDLRLDPGES KSYSYSEVLP IEGPPSFRGQ SVKYVYKLTI GCQRVNSPIT LLRVPLRVLV LTGLQDVRFP QDEAVAPSSP FLEEDEGGKK DSWLAELAGE RLMAATSCRS LHLYNISDGR GKVGTFGIFK SVYRLGEDVV GTLNLGEGTV ACLQFSVSLQ TEERVQPEYQ RRRGAGGVPS VSHVTHARHQ ESCLHTTRTS FSLPIPLSST PGFCTAIVSL KWRLHFEFVT SREPGLVLLP PVEQPEPTTW TGPEQVPVDT FSWDLPIKVL PTSPTLASYA APGPSTSTIT I
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: RGP1 (RGP1 Retrograde Golgi Transport Homolog (RGP1))

Andere Bezeichnung: RGP1 (RGP1 Produkte)

Synonyme: RGP1 Protein, zgc:92283 Protein, MGC80288 Protein, KIAA0258 Protein, 1110029E03Rik Protein, AI527196 Protein, F730001J03 Protein, mKIAA0258 Protein, RGD1311757 Protein, RAB6A-GEF complex partner protein 2 Protein, RGP1 homolog, RAB6A GEF complex partner 1 Protein, GP1 homolog, RAB6A GEF complex partner 1 Protein, RGP1 homolog, RAB6A GEF complex partner 1 L homeolog Protein, RAB6A GEF compex partner 1 Protein, LOC413244 Protein, RGP1 Protein, rgp1 Protein, LOC100643245 Protein, rgp1.L Protein, Rgp1 Protein

Hintergrund: RAB6A-GEF complex partner protein 2 (Retrograde Golgi transport protein RGP1 homolog),FUNCTION: The RIC1-RGP1 complex acts as a guanine nucleotide exchange factor (GEF), which activates RAB6A by exchanging bound GDP for free GTP and may thereby required for efficient fusion of endosome-derived vesicles with the Golgi compartment. The RIC1-RGP1 complex participates in the recycling of mannose-6-phosphate receptors. {ECO:0000269|PubMed:23091056}.

Molekulargewicht: 42.5 kDa

UniProt: Q92546