RBP1 Protein (AA 2-135) (His tag)
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- Target Alle RBP1 Proteine anzeigen
- RBP1 (Retinol Binding Protein 1, Cellular (RBP1))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 2-135
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses RBP1 Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- Sequenz
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PVDFTGYWKM LVNENFEEYL RALDVNVALR KIANLLKPDK EIVQDGDHMI IRTLSTFRNY IMDFQVGKEF EEDLTGIDDR KCMTTVSWDG DKLQCVQKGE KEGRGWTQWI EGDELHLEMR VEGVVCKQVF KKVQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human RBP1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product RBP1 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- RBP1 (Retinol Binding Protein 1, Cellular (RBP1))
- Andere Bezeichnung
- RBP1 (RBP1 Produkte)
- Synonyme
- RBBP-1 Protein, RBBP1 Protein, RBP-1 Protein, RBP1 Protein, CRABP-I Protein, CRBP Protein, CRBP1 Protein, CRBPI Protein, RBPC Protein, cb465 Protein, rbp1 Protein, wu:fb75e07 Protein, zgc:73335 Protein, CRABP1 Protein, Crbp Protein, Rbp-1 Protein, rbp1b Protein, sb:eu611 Protein, zgc:100825 Protein, AT-rich interaction domain 4A Protein, retinol binding protein 1 Protein, retinol binding protein 1a, cellular Protein, retinol binding protein 1 L homeolog Protein, retinol binding protein 1, cellular Protein, retinol binding protein 1b, cellular Protein, ARID4A Protein, RBP1 Protein, rbp5 Protein, rbp1.L Protein, rbp1 Protein, Rbp1 Protein
- Hintergrund
- Intracellular transport of retinol.
- Molekulargewicht
- 16.7 kDa Including tag.
- UniProt
- P09455
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