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RAB6A Protein (AA 2-208) (His tag)

Crystallography grade RAB6A Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Produktnummer ABIN3086873
  • Target Alle RAB6A Proteine anzeigen
    RAB6A (RAB6A, Member RAS Oncogene Family (RAB6A))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-208
    Spezies
    • 7
    • 1
    • 1
    • 1
    Human
    Quelle
    • 4
    • 2
    • 2
    • 2
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses RAB6A Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)
    Sequenz
    STGGDFGNPL RKFKLVFLGE QSVGKTSLIT RFMYDSFDNT YQATIGIDFL SKTMYLEDRT VRLQLWDTAG QERFRSLIPS YIRDSTVAVV VYDITNVNSF QQTTKWIDDV RTERGSDVII MLVGNKTDLA DKRQVSIEEG ERKAKELNVM FIETSAKAGY NVKQLFRRVA AALPGMESTQ DRSREDMIDI KLEKPQEQPV SEGGCSC
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human RAB6A Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product RAB6A Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    RAB6A (RAB6A, Member RAS Oncogene Family (RAB6A))
    Andere Bezeichnung
    RAB6A (RAB6A Produkte)
    Synonyme
    fb43c01 Protein, wu:fb43c01 Protein, zgc:55788 Protein, rab6 Protein, MGC53077 Protein, rab6b Protein, rab6a' Protein, MGC76176 Protein, LOC692966 Protein, RAB6 Protein, 2610028L11Rik Protein, AA419671 Protein, Rab6 Protein, RCO4-3 Protein, Rab6b Protein, RAB6A, member RAS oncogene family Protein, RAB6A, member RAS oncogene family S homeolog Protein, ras small monomeric GTPase Rab6 Protein, ras-related protein Rab-6A Protein, rab6a Protein, rab6a.S Protein, LOC692966 Protein, RAB6A Protein, Rab6a Protein
    Hintergrund
    Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity.
    Molekulargewicht
    24.4 kDa Including tag.
    UniProt
    P20340
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