PTPN4 Protein (AA 1-926) (Strep Tag)

Produktnummer ABIN3086688

Produktdetails

Target: PTPN4 (Tyrosine-Protein Phosphatase Non-Receptor Type 4 (PTPN4))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-926

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses PTPN4 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MTSRFRLPAG RTYNVRASEL ARDRQHTEVV CNILLLDNTV QAFKVNKHDQ GQVLLDVVFK HLDLTEQDYF GLQLADDSTD NPRWLDPNKP IRKQLKRGSP YSLNFRVKFF VSDPNKLQEE YTRYQYFLQI KQDILTGRLP CPSNTAALLA SFAVQSELGD YDQSENLSGY LSDYSFIPNQ PQDFEKEIAK LHQQHIGLSP AEAEFNYLNT ARTLELYGVE FHYARDQSNN EIMIGVMSGG ILIYKNRVRM NTFPWLKIVK ISFKCKQFFI QLRKELHESR ETLLGFNMVN YRACKNLWKA CVEHHTFFRL DRPLPPQKNF FAHYFTLGSK FRYCGRTEVQ SVQYGKEKAN KDRVFARSPS KPLARKLMDW EVVSRNSISD DRLETQSLPS RSPPGTPNHR NSTFTQEGTR LRPSSVGHLV DHMVHTSPSE VFVNQRSPSS TQANSIVLES SPSQETPGDG KPPALPPKQS KKNSWNQIHY SHSQQDLESH INETFDIPSS PEKPTPNGGI PHDNLVLIRM KPDENGRFGF NVKGGYDQKM PVIVSRVAPG TPADLCVPRL NEGDQVVLIN GRDIAEHTHD QVVLFIKASC ERHSGELMLL VRPNAVYDVV EEKLENEPDF QYIPEKAPLD SVHQDDHSLR ESMIQLAEGL ITGTVLTQFD QLYRKKPGMT MSCAKLPQNI SKNRYRDISP YDATRVILKG NEDYINANYI NMEIPSSSII NQYIACQGPL PHTCTDFWQM TWEQGSSMVV MLTTQVERGR VKCHQYWPEP TGSSSYGCYQ VTCHSEEGNT AYIFRKMTLF NQEKNESRPL TQIQYIAWPD HGVPDDSSDF LDFVCHVRNK RAGKEEPVVV HCSAGIGRTG VLITMETAMC LIECNQPVYP LDIVRTMRDQ RAMMIQTPSQ YRFVCEAILK VYEEGFVKPL TTSTNK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PTPN4 (Tyrosine-Protein Phosphatase Non-Receptor Type 4 (PTPN4))

Andere Bezeichnung: PTPN4 (PTPN4 Produkte)

Synonyme: MEG Protein, PTPMEG Protein, PTPMEG1 Protein, Ptn4 Protein, TEP Protein, TEP/mPTPMEG Protein, hPTP-MEG Protein, ptpn4 Protein, si:busm1-37o5.1 Protein, si:dz37o5.1 Protein, protein tyrosine phosphatase, non-receptor type 4 Protein, tyrosine-protein phosphatase non-receptor type 4 Protein, protein tyrosine phosphatase, non-receptor type 4a Protein, PTPN4 Protein, Ptpn4 Protein, CpipJ_CPIJ003699 Protein, Tsp_12341 Protein, Tsp_14272 Protein, ptpn4a Protein

Hintergrund: Tyrosine-protein phosphatase non-receptor type 4 (EC 3.1.3.48) (Protein-tyrosine phosphatase MEG1) (MEG) (PTPase-MEG1),FUNCTION: Phosphatase that plays a role in immunity, learning, synaptic plasticity or cell homeostasis (PubMed:25825441, PubMed:27246854). Regulates neuronal cell homeostasis by protecting neurons against apoptosis (PubMed:20086240). Negatively regulates TLR4-induced interferon beta production by dephosphorylating adapter TICAM2 and inhibiting subsequent TRAM-TRIF interaction (PubMed:25825441). Dephosphorylates also the immunoreceptor tyrosine-based activation motifs/ITAMs of the TCR zeta subunit and thereby negatively regulates TCR-mediated signaling pathway (By similarity). May act at junctions between the membrane and the cytoskeleton. {ECO:0000250, ECO:0000250|UniProtKB:Q9WU22, ECO:0000269|PubMed:20086240, ECO:0000269|PubMed:25825441, ECO:0000269|PubMed:27246854}.

Molekulargewicht: 105.9 kDa

UniProt: P29074