Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

PIGS Protein (AA 40-520) (His tag)

Crystallography grade PIGS Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3086091
  • Target Alle PIGS Proteine anzeigen
    PIGS (Phosphatidylinositol Glycan Anchor Biosynthesis, Class S (PIGS))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 40-520
    Spezies
    • 3
    • 2
    Human
    Quelle
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses PIGS Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    ETYRASLPYS QISGLNALQL RLMVPVTVVF TRESVPLDDQ EKLPFTVVHE REIPLKYKMK IKCRFQKAYR RALDHEEEAL SSGSVQEAEA MLDEPQEQAE GSLTVYVISE HSSLLPQDMM SYIGPKRTAV VRGIMHREAF NIIGRRIVQV AQAMSLTEDV LAAALADHLP EDKWSAEKRR PLKSSLGYEI TFSLLNPDPK SHDVYWDIEG AVRRYVQPFL NALGAAGNFS VDSQILYYAM LGVNPRFDSA SSSYYLDMHS LPHVINPVES RLGSSAASLY PVLNFLLYVP ELAHSPLYIQ DKDGAPVATN AFHSPRWGGI MVYNVDSKTY NASVLPVRVE VDMVRVMEVF LAQLRLLFGI AQPQLPPKCL LSGPTSEGLM TWELDRLLWA RSVENLATAT TTLTSLAQLL GKISNIVIKD DVASEVYKAV AAVQKSAEEL ASGHLASAFV ASQEAVTSSE LAFFDPSLLH LLYFPDDQKF A
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PIGS Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product PIGS Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    PIGS (Phosphatidylinositol Glycan Anchor Biosynthesis, Class S (PIGS))
    Andere Bezeichnung
    PIGS (PIGS Produkte)
    Synonyme
    BC058979 Protein, Gm393 Protein, Gm689 Protein, phosphatidylinositol glycan anchor biosynthesis class S Protein, phosphatidylinositol glycan anchor biosynthesis, class S Protein, PIGS Protein, Pigs Protein
    Hintergrund
    Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates. {ECO:0000269|PubMed:11483512}.
    Molekulargewicht
    54.4 kDa Including tag.
    UniProt
    Q96S52
    Pathways
    Inositol Metabolic Process, SARS-CoV-2 Protein Interaktom
Sie sind hier:
Kundenservice