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GSTZ1 Protein (AA 1-216) (His tag)

Crystallography grade GSTZ1 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Produktnummer ABIN3083310
  • Target Alle GSTZ1 Proteine anzeigen
    GSTZ1 (Glutathione Transferase zeta 1 (Maleylacetoacetate Isomerase) (GSTZ1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-216
    Spezies
    • 6
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 4
    • 3
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses GSTZ1 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequenz
    MQAGKPILYS YFRSSCSWRV RIALALKGID YKTVPINLIK DRGQQFSKDF QALNPMKQVP TLKIDGITIH QSLAIIEYLE EMRPTPRLLP QDPKKRASVR MISDLIAGGI QPLQNLSVLK QVGEEMQLTW AQNAITCGFN ALEQILQSTA GIYCVGDEVT MADLCLVPQV ANAERFKVDL TPYPTISSIN KRLLVLEAFQ VSHPCRQPDT PTELRA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human GSTZ1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product GSTZ1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    GSTZ1 (Glutathione Transferase zeta 1 (Maleylacetoacetate Isomerase) (GSTZ1))
    Andere Bezeichnung
    GSTZ1 (GSTZ1 Produkte)
    Synonyme
    GSTZ1-1 Protein, MAAI Protein, MAI Protein, zgc:113898 Protein, zgc:92869 Protein, PSPTO3554 Protein, DDBDRAFT_0204466 Protein, DDBDRAFT_0231608 Protein, DDB_0204466 Protein, DDB_0231608 Protein, CG9362 Protein, Dmel\CG9362 Protein, gstz1 Protein, glutathione S-transferase zeta 1 Protein, glutathione transferase zeta 1 (maleylacetoacetate isomerase) Protein, maleylacetoacetate isomerase Protein, Glutathione S transferase Z1 Protein, glutathione S-transferase zeta 1 L homeolog Protein, GSTZ1 Protein, Gstz1 Protein, gstz1 Protein, maiA Protein, hmgC Protein, Patl_1448 Protein, Pnap_2807 Protein, Bind_0562 Protein, Bphyt_0577 Protein, Dtpsy_1678 Protein, Hbal_2638 Protein, mai Protein, BC1002_0299 Protein, Fbal_2576 Protein, Mesop_3937 Protein, GstZ1 Protein, gstz1.L Protein
    Hintergrund
    Bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid. {ECO:0000269|PubMed:10739172}.
    Molekulargewicht
    25.2 kDa Including tag.
    UniProt
    O43708
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