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LGI1 Protein (AA 35-557) (His tag)

Crystallography grade LGI1 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3083026
  • Target Alle LGI1 Proteine anzeigen
    LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 35-557
    Spezies
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 2
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses LGI1 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    KKPAKPKCPA VCTCTKDNAL CENARSIPRT VPPDVISLSF VRSGFTEISE GSFLFTPSLQ LLLFTSNSFD VISDDAFIGL PHLEYLFIEN NNIKSISRHT FRGLKSLIHL SLANNNLQTL PKDIFKGLDS LTNVDLRGNS FNCDCKLKWL VEWLGHTNAT VEDIYCEGPP EYKKRKINSL SSKDFDCIIT EFAKSQDLPY QSLSIDTFSY LNDEYVVIAQ PFTGKCIFLE WDHVEKTFRN YDNITGTSTV VCKPIVIETQ LYVIVAQLFG GSHIYKRDSF ANKFIKIQDI EILKIRKPND IETFKIENNW YFVVADSSKA GFTTIYKWNG NGFYSHQSLH AWYRDTDVEY LEIVRTPQTL RTPHLILSSS SQRPVIYQWN KATQLFTNQT DIPNMEDVYA VKHFSVKGDV YICLTRFIGD SKVMKWGGSS FQDIQRMPSR GSMVFQPLQI NNYQYAILGS DYSFTQVYNW DAEKAKFVKF QELNVQAPRS FTHVSINKRN FLFASSFKGN TQIYKHVIVD LSA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human LGI1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product LGI1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))
    Andere Bezeichnung
    LGI1 (LGI1 Produkte)
    Synonyme
    ADLTE Protein, ADPAEF Protein, ADPEAF Protein, EPITEMPIN Protein, EPT Protein, ETL1 Protein, IB1099 Protein, BB130740 Protein, MGC146600 Protein, LGIa Protein, fb78h12 Protein, wu:fb78h12 Protein, zgc:65886 Protein, leucine rich glioma inactivated 1 Protein, leucine-rich repeat LGI family, member 1 Protein, leucine-rich, glioma inactivated 1 Protein, leucine-rich, glioma inactivated 1b Protein, leucine-rich, glioma inactivated 1a Protein, LGI1 Protein, Lgi1 Protein, lgi1 Protein, lgi1b Protein, lgi1a Protein
    Hintergrund
    Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival. {ECO:0000250, ECO:0000269|PubMed:15047712, ECO:0000269|PubMed:16518856}.
    Molekulargewicht
    61.0 kDa Including tag.
    UniProt
    O95970
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