CKB Protein (AA 2-381) (His tag)
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- Target Alle CKB Proteine anzeigen
- CKB (Creatine Kinase, Brain (CKB))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 2-381
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Spezies
- Human
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses CKB Protein ist gelabelt mit His tag.
- Applikation
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequenz
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PFSNSHNALK LRFPAEDEFP DLSAHNNHMA KVLTPELYAE LRAKSTPSGF TLDDVIQTGV DNPGHPYIMT VGCVAGDEES YEVFKDLFDP IIEDRHGGYK PSDEHKTDLN PDNLQGGDDL DPNYVLSSRV RTGRSIRGFC LPPHCSRGER RAIEKLAVEA LSSLDGDLAG RYYALKSMTE AEQQQLIDDH FLFDKPVSPL LLASGMARDW PDARGIWHND NKTFLVWVNE EDHLRVISMQ KGGNMKEVFT RFCTGLTQIE TLFKSKDYEF MWNPHLGYIL TCPSNLGTGL RAGVHIKLPN LGKHEKFSEV LKRLRLQKRG TGGVDTAAVG GVFDVSNADR LGFSEVELVQ MVVDGVKLLI EMEQRLEQGQ AIDDLMPAQK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human CKB Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- CKB (Creatine Kinase, Brain (CKB))
- Andere Bezeichnung
- CKB (CKB Produkte)
- Synonyme
- B-CK Protein, CKBB Protein, b-ck Protein, ckbb Protein, CKB Protein, Bck Protein, Ck-3 Protein, Ck3 Protein, Ckbb Protein, bck Protein, ckb Protein, cb594 Protein, wu:fj36f10 Protein, wu:fj37d11 Protein, Ckbr Protein, mg:cb02h12 Protein, wu:fb62d04 Protein, wu:fc14g11 Protein, zgc:154095 Protein, zgc:92079 Protein, creatine kinase B Protein, creatine kinase, brain L homeolog Protein, creatine kinase B-type Protein, creatine kinase, brain Protein, creatine kinase, brain b Protein, creatine kinase, brain a Protein, CKB Protein, ckb.L Protein, Ckb Protein, ckb Protein, ckbb Protein, ckba Protein
- Hintergrund
- Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
- Molekulargewicht
- 43.5 kDa Including tag.
- UniProt
- P12277
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