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KRT73 Protein (AA 1-540) (His tag)

Crystallography grade KRT73 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3082134
  • Target Alle KRT73 Produkte
    KRT73 (Keratin 73 (KRT73))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-540
    Spezies
    Human
    Quelle
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses KRT73 Protein ist gelabelt mit His tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MSRQFTYKSG AAAKGGFSGC SAVLSGGSSS SYRAGGKGLS GGFSSRSLYS LGGARSISFN VASGSGWAGG YGFGRGRASG FAGSMFGSVA LGSVCPSLCP PGGIHQVTIN KSLLAPLNVE LDPEIQKVRA QEREQIKVLN NKFASFIDKV RFLEQQNQVL ETKWELLQQL DLNNCKNNLE PILEGYISNL RKQLETLSGD RVRLDSELRS VREVVEDYKK RYEEEINKRT TAENEFVVLK KDVDAAYTSK VELQAKVDAL DGEIKFFKCL YEGETAQIQS HISDTSIILS MDNNRNLDLD SIIAEVRAQY EEIARKSKAE AEALYQTKFQ ELQLAAGRHG DDLKHTKNEI SELTRLIQRL RSEIESVKKQ CANLETAIAD AEQRGDCALK DARAKLDELE GALQQAKEEL ARMLREYQEL LSVKLSLDIE IATYRKLLEG EECRMSGEYT NSVSISVINS SMAGMAGTGA GFGFSNAGTY GYWPSSVSGG YSMLPGGCVT GSGNCSPRGE ARTRLGSASE FRDSQGKTLA LSSPTKKTMR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human KRT73 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    KRT73 (Keratin 73 (KRT73))
    Andere Bezeichnung
    KRT73 (KRT73 Produkte)
    Synonyme
    IRT6IRS3 Protein, K6IRS3 Protein, KRT6IRS3 Protein, BC067067 Protein, Kb36 Protein, keratin 73 Protein, keratin, type II cytoskeletal 73 Protein, KRT73 Protein, LOC699452 Protein, LOC100092881 Protein, LOC100347647 Protein, LOC100398973 Protein, LOC100511079 Protein, Krt73 Protein
    Hintergrund
    Has a role in hair formation. Specific component of keratin intermediate filaments in the inner root sheath (IRS) of the hair follicle (Probable). {ECO:0000305}.
    Molekulargewicht
    59.9 kDa Including tag.
    UniProt
    Q86Y46
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