EIF2S2 Protein (AA 2-333) (His tag)

Produktnummer ABIN3081777

Produktdetails

Target: EIF2S2 (Eukaryotic Translation Initiation Factor 2, Subunit 2 Beta, 38kDa (EIF2S2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-333

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses EIF2S2 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: SGDEMIFDPT MSKKKKKKKK PFMLDEEGDT QTEETQPSET KEVEPEPTED KDLEADEEDT RKKDASDDLD DLNFFNQKKK KKKTKKIFDI DEAEEGVKDL KIESDVQEPT EPEDDLDIML GNKKKKKKNV KFPDEDEILE KDEALEDEDN KKDDGISFSN QTGPAWAGSE RDYTYEELLN RVFNIMREKN PDMVAGEKRK FVMKPPQVVR VGTKKTSFVN FTDICKLLHR QPKHLLAFLL AELGTSGSID GNNQLVIKGR FQQKQIENVL RRYIKEYVTC HTCRSPDTIL QKDTRLYFLQ CETCHSRCSV ASIKTGFQAV TGKRAQLRAK AN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human EIF2S2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: EIF2S2 (Eukaryotic Translation Initiation Factor 2, Subunit 2 Beta, 38kDa (EIF2S2))

Andere Bezeichnung: EIF2S2 (EIF2S2 Produkte)

Synonyme: EIF2 Protein, EIF2B Protein, EIF2beta Protein, PPP1R67 Protein, eIF-2-beta Protein, 2810026E11Rik Protein, 38kDa Protein, AA408636 Protein, AA571381 Protein, AA986487 Protein, AW822225 Protein, D2Ertd303e Protein, zgc:77084 Protein, wu:fc26g03 Protein, wu:fu10f07 Protein, MGC130959 Protein, DDBDRAFT_0205105 Protein, DDBDRAFT_0234112 Protein, DDB_0205105 Protein, DDB_0234112 Protein, AO090005001412 Protein, eukaryotic translation initiation factor 2 subunit beta Protein, eukaryotic translation initiation factor 2, subunit 2 (beta) Protein, eukaryotic translation initiation factor 2, subunit 2 beta Protein, eukaryotic translation initiation factor 2 subunit beta L homeolog Protein, eIF-3 beta Protein, hypothetical protein Protein, eukaryotic translation initiation factor 2 subunit 2 Protein, EIF2S2 Protein, Eif2s2 Protein, eif2s2 Protein, eif2s2.L Protein, eIF2s2 Protein, TP02_0678 Protein, EDI_093670 Protein, AOR_1_2450174 Protein, MGYG_05853 Protein, PGTG_10708 Protein, LOC101111733 Protein

Hintergrund: EIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Molekulargewicht: 39.2 kDa Including tag.

UniProt: P20042