IL27 Receptor alpha Protein (AA 33-516) (His tag)

Produktnummer ABIN3081771

Produktdetails

Target: IL27 Receptor alpha (IL27RA) (Interleukin 27 Receptor, alpha (IL27RA))

Protein-Typ: Recombinant

Proteineigenschaft: AA 33-516

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses IL27 Receptor alpha Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: QGSAGPLQCY GVGPLGDLNC SWEPLGDLGA PSELHLQSQK YRSNKTQTVA VAAGRSWVAI PREQLTMSDK LLVWGTKAGQ PLWPPVFVNL ETQMKPNAPR LGPDVDFSED DPLEATVHWA PPTWPSHKVL ICQFHYRRCQ EAAWTLLEPE LKTIPLTPVE IQDLELATGY KVYGRCRMEK EEDLWGEWSP ILSFQTPPSA PKDVWVSGNL CGTPGGEEPL LLWKAPGPCV QVSYKVWFWV GGRELSPEGI TCCCSLIPSG AEWARVSAVN ATSWEPLTNL SLVCLDSASA PRSVAVSSIA GSTELLVTWQ PGPGEPLEHV VDWARDGDPL EKLNWVRLPP GNLSALLPGN FTVGVPYRIT VTAVSASGLA SASSVWGFRE ELAPLVGPTL WRLQDAPPGT PAIAWGEVPR HQLRGHLTHY TLCAQSGTSP SVCMNVSGNT QSVTLPDLPW GPCELWVTAS TIAGQGPPGP ILRLHLPDNT LRWK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human IL27RA Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: IL27 Receptor alpha (IL27RA) (Interleukin 27 Receptor, alpha (IL27RA))

Andere Bezeichnung: IL27RA (IL27RA Produkte)

Synonyme: IL27RA Protein, CRL1 Protein, IL-27RA Protein, IL27R Protein, TCCR Protein, WSX1 Protein, zcytor1 Protein, IL-27R Protein, Tccr Protein, WSX-1 Protein, Wsx1 Protein, interleukin 27 receptor subunit alpha Protein, interleukin-27 receptor subunit alpha Protein, interleukin 27 receptor, alpha Protein, IL27RA Protein, LOC484907 Protein, Il27ra Protein

Hintergrund: Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms. {ECO:0000269|PubMed:14764690}.

Molekulargewicht: 53.5 kDa Including tag.

UniProt: Q6UWB1

Pathways: Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response