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HMGB3 Protein (AA 2-200) (His tag)

Crystallography grade HMGB3 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Produktnummer ABIN3081510
  • Target Alle HMGB3 Proteine anzeigen
    HMGB3 (High Mobility Group Box 3 (HMGB3))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-200
    Spezies
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 4
    • 3
    • 3
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses HMGB3 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequenz
    AKGDPKKPKG KMSAYAFFVQ TCREEHKKKN PEVPVNFAEF SKKCSERWKT MSGKEKSKFD EMAKADKVRY DREMKDYGPA KGGKKKKDPN APKRPPSGFF LFCSEFRPKI KSTNPGISIG DVAKKLGEMW NNLNDSEKQP YITKAAKLKE KYEKDVADYK SKGKFDGAKG PAKVARKKVE EEDEEEEEEE EEEEEEEDE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human HMGB3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product HMGB3 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    HMGB3 (High Mobility Group Box 3 (HMGB3))
    Andere Bezeichnung
    HMGB3 (HMGB3 Produkte)
    Synonyme
    HMG-2a Protein, HMG-4 Protein, HMG2A Protein, HMG4 Protein, Hmg2a Protein, Hmg4 Protein, RGD1564407 Protein, hmgb3 Protein, MGC54022 Protein, Xhmgb3 Protein, MGC88931 Protein, fa19b06 Protein, fj43d02 Protein, wu:fa19b06 Protein, wu:fj43d02 Protein, zgc:112073 Protein, HMGB3 Protein, NFD03 Protein, NFD3 Protein, NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR Protein, high mobility group B3 Protein, HMG-1 Protein, HMG2a Protein, HMGB1 Protein, high mobility group box 3 Protein, high mobility group box 3b Protein, high mobility group box 3 S homeolog Protein, high mobility group protein Protein, high mobility group B3 Protein, High mobility group protein B3 Protein, HMGB3 Protein, Hmgb3 Protein, hmgb3 Protein, hmgb3b Protein, hmgb3.S Protein
    Hintergrund
    Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity). {ECO:0000250|UniProtKB:O54879, ECO:0000250|UniProtKB:P09429, ECO:0000250|UniProtKB:P40618}.
    Molekulargewicht
    23.8 kDa Including tag.
    UniProt
    O15347
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