HAT1 Protein (AA 2-419) (His tag)

Produktnummer ABIN3081304

Produktdetails

Target: HAT1 (Histone Acetyltransferase 1 (HAT1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-419

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses HAT1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: AGFGAMEKFL VEYKSAVEKK LAEYKCNTNT AIELKLVRFP EDLENDIRTF FPEYTHQLFG DDETAFGYKG LKILLYYIAG SLSTMFRVEY ASKVDENFDC VEADDVEGKI RQIIPPGFCT NTNDFLSLLE KEVDFKPFGT LLHTYSVLSP TGGENFTFQI YKADMTCRGF REYHERLQTF LMWFIETASF IDVDDERWHY FLVFEKYNKD GATLFATVGY MTVYNYYVYP DKTRPRVSQM LILTPFQGQG HGAQLLETVH RYYTEFPTVL DITAEDPSKS YVKLRDFVLV KLCQDLPCFS REKLMQGFNE DMAIEAQQKF KINKQHARRV YEILRLLVTD MSDAEQYRSY RLDIKRRLIS PYKKKQRDLA KMRKCLRPEE LTNQMNQIEI SMQHEQLEES FQELVEDYRR VIERLAQE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human HAT1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: HAT1 (Histone Acetyltransferase 1 (HAT1))

Andere Bezeichnung: HAT1 (HAT1 Produkte)

Synonyme: KAT1 Protein, HAC07 Protein, HAC7 Protein, HAG02 Protein, MIK19.19 Protein, MIK19_19 Protein, histone acetyltransferase of the GNAT family 2 Protein, hac106 Protein, hat1 Protein, zgc:92639 Protein, histone acetyltransferase 1 Protein, histone acetyltransferase of the GNAT family 2 Protein, histone acetyltransferase catalytic subunit Protein, histone acetyltransferase Hat1 Protein, histone acetyltransferase 1 L homeolog Protein, HAT1 Protein, Hat1 Protein, HAG2 Protein, hat1 Protein, hat1.L Protein

Hintergrund: Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage. {ECO:0000269|PubMed:11585814, ECO:0000269|PubMed:20953179, ECO:0000269|PubMed:22615379, ECO:0000269|PubMed:9427644}.

Molekulargewicht: 50.3 kDa Including tag.

UniProt: O14929