Glutaminase Protein (GLS) (AA 17-669) (His tag)

Produktnummer ABIN3080857

Produktdetails

Target: Glutaminase (GLS)

Protein-Typ: Recombinant

Proteineigenschaft: AA 17-669

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses Glutaminase Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: SPAGVSATLR RAQPLVTLCR RPRGGGRPAA GPAAAARLHP WWGGGGWPAE PLARGLSSSP SEILQELGKG STHPQPGVSP PAAPAAPGPK DGPGETDAFG NSEGKELVAS GENKIKQGLL PSLEDLLFYT IAEGQEKIPV HKFITALKST GLRTSDPRLK ECMDMLRLTL QTTSDGVMLD KDLFKKCVQS NIVLLTQAFR RKFVIPDFMS FTSHIDELYE SAKKQSGGKV ADYIPQLAKF SPDLWGVSVC TVDGQRHSTG DTKVPFCLQS CVKPLKYAIA VNDLGTEYVH RYVGKEPSGL RFNKLFLNED DKPHNPMVNA GAIVVTSLIK QGVNNAEKFD YVMQFLNKMA GNEYVGFSNA TFQSERESGD RNFAIGYYLK EKKCFPEGTD MVGILDFYFQ LCSIEVTCES ASVMAATLAN GGFCPITGER VLSPEAVRNT LSLMHSCGMY DFSGQFAFHV GLPAKSGVAG GILLVVPNVM GMMCWSPPLD KMGNSVKGIH FCHDLVSLCN FHNYDNLRHF AKKLDPRREG GDQRVKSVIN LLFAAYTGDV SALRRFALSA MDMEQRDYDS RTALHVAAAE GHVEVVKFLL EACKVNPFPK DRWNNTPMDE ALHFGHHDVF KILQEYQVQY TPQGDSDNGK ENQTVHKNLD GLL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human GLS Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Glutaminase (GLS)

Andere Bezeichnung: GLS (GLS Produkte)

Synonyme: Glut Protein, RATGLUT Protein, gls Protein, si:dz87i4.2 Protein, wu:fa97e05 Protein, GA Protein, PAG Protein, AAD20 Protein, GAC Protein, GAM Protein, GLS1 Protein, KGA Protein, AI314027 Protein, 6330442B14 Protein, B230365M23Rik Protein, CG8772 Protein, CG8872 Protein, Dmel\\CG42708 Protein, Dmel_CG8772 Protein, BA3155 Protein, glutaminase Protein, glutaminase b Protein, Glutaminase Protein, glutaminase kidney isoform, mitochondrial Protein, Gls Protein, GLS Protein, gls Protein, glsb Protein, glsA2 Protein, glsA-2 Protein, Arnit_3113 Protein, Celal_2913 Protein, Celly_0289 Protein, Weevi_2101 Protein, Mesop_4683 Protein, LOC100069617 Protein

Hintergrund: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.

Molekulargewicht: 72.5 kDa Including tag.

UniProt: O94925

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effekt