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GFAP Protein (AA 1-432) (His tag)

Crystallography grade GFAP Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3080596
  • Target Alle GFAP Proteine anzeigen
    GFAP (Glial Fibrillary Acidic Protein (GFAP))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-432
    Spezies
    • 10
    • 7
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 16
    • 5
    • 3
    • 2
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses GFAP Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    MHHHHHHERR RITSAARRSY VSSGEMMVGG LAPGRRLGPG TRLSLARMPP PLPTRVDFSL AG ALNA GFKETRASER AEMMELNDRF ASYIEKVRFL EQQNKALAAE LNQLRAKEPT KLADVYQAEL RELRLRLDQL TANSARLEVE RDNLAQDLAT VRQKLQDETN LRLEAENNLA AYRQEADEAT LARLDLERKI ESLEEEIRFL RKIHEEEVRE LQEQLARQQV HVELDVAKPD LTAALKEIRT QYEAMASSNM HEAEEWYRSK FADLTDAAAR NAELLRQAKH EANDYRRQLQ SLTCDLESLR GTNESLERQM REQEERHVRE AASYQEALAR LEEEGQSLKD EMARHLQEYQ DLLNVKLALD IEIATYRKLL EGEENRITIP VQTFSNLQIR ETSLDTKSVS EGHLKRNIVV KTVEMRDGEV IKESKQEHKD VM
    Sequence includes N-terminal His-tag
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PLP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    The protein is purified from the cleared cell lysate using EDTA and DTT resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    In solution (20 mM Hepes, pH 7.4, 100 mM NaCl, 2 mM EDTA)
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    GFAP (Glial Fibrillary Acidic Protein (GFAP))
    Andere Bezeichnung
    GFAP (GFAP Produkte)
    Synonyme
    GFAP Protein, AI836096 Protein, cb345 Protein, etID36982.3 Protein, gfapl Protein, wu:fb34h11 Protein, wu:fk42c12 Protein, xx:af506734 Protein, zgc:110485 Protein, glial fibrillary acidic protein Protein, GFAP Protein, LOC100136168 Protein, gfap Protein, Gfap Protein
    Hintergrund
    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
    Molekulargewicht
    50.8 kDa Including tag
    UniProt
    P14136
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