Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

FAM25B Protein (AA 1-89) (His tag)

Crystallography grade FAM25B Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3080192
  • Target
    FAM25B (Family with Sequence Similarity 25, Member B (FAM25B))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-89
    Spezies
    Human
    Quelle
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses FAM25B Protein ist gelabelt mit His tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MLGGLGKLAA EGLAHRTEKA TEGAIHAVEE VVKEVVGHAK ETGEKAIAEA IKKAQESGDK KMKEITETVT NTVTNAITHA AESLDKLGQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human FAM25BP Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    FAM25B (Family with Sequence Similarity 25, Member B (FAM25B))
    Andere Bezeichnung
    FAM25BP
    Synonyme
    FAM25A Protein, FAM25C Protein, FAM25G Protein, protein FAM25 Protein, FAM25BP Protein
    Molekulargewicht
    10.3 kDa Including tag.
    UniProt
    B3EWG4
Sie sind hier:
Kundenservice