EXOC5 Protein (AA 2-708) (His tag)

Produktnummer ABIN3079578

Produktdetails

Target: EXOC5 (Exocyst Complex Component 5 (EXOC5))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-708

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses EXOC5 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: ATTAELFEEP FVADEYIERL VWRTPGGGSR GGPEAFDPKR LLEEFVNHIQ ELQIMDERIQ RKVEKLEQQC QKEAKEFAKK VQELQKSNQV AFQHFQELDE HISYVATKVC HLGDQLEGVN TPRQRAVEAQ KLMKYFNEFL DGELKSDVFT NSEKIKEAAD IIQKLHLIAQ ELPFDRFSEV KSKIASKYHD LECQLIQEFT SAQRRGEISR MREVAAVLLH FKGYSHCVDV YIKQCQEGAY LRNDIFEDAG ILCQRVNKQV GDIFSNPETV LAKLIQNVFE IKLQSFVKEQ LEECRKSDAE QYLKNLYDLY TRTTNLSSKL MEFNLGTDKQ TFLSKLIKSI FISYLENYIE VETGYLKSRS AMILQRYYDS KNHQKRSIGT GGIQDLKERI RQRTNLPLGP SIDTHGETFL SQEVVVNLLQ ETKQAFERCH RLSDPSDLPR NAFRIFTILV EFLCIEHIDY ALETGLAGIP SSDSRNANLY FLDVVQQANT IFHLFDKQFN DHLMPLISSS PKLSECLQKK KEIIEQMEMK LDTGIDRTLN CMIGQMKHIL AAEQKKTDFK PEDENNVLIQ YTNACVKVCA YVRKQVEKIK NSMDGKNVDT VLMELGVRFH RLIYEHLQQY SYSCMGGMLA ICDVAEYRKC AKDFKIPMVL HLFDTLHALC NLLVVAPDNL KQVCSGEQLA NLDKNILHSF VQLRADYRSA RLARHFS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human EXOC5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: EXOC5 (Exocyst Complex Component 5 (EXOC5))

Andere Bezeichnung: EXOC5 (EXOC5 Produkte)

Synonyme: CG6159 Protein, Dmel\\CG6159 Protein, P71 Protein, Sec10 Protein, Sec10p Protein, dSec10 Protein, dsec10 Protein, SEC10L1 Protein, MGC80624 Protein, sec10l1 Protein, zgc:175220 Protein, HSEC10 Protein, PRO1912 Protein, SEC10 Protein, SEC10P Protein, AI447711 Protein, AI448003 Protein, Gm76 Protein, Sec10l1 Protein, exocyst complex component sec10 Protein, Secretory 10 Protein, exocyst complex component 5 Protein, exocyst complex component 5 L homeolog Protein, exocyst complex component sec10 Protein, Exocyst complex component 5 Protein, Sec10 Protein, EXOC5 Protein, exoc5.L Protein, exoc5 Protein, CpipJ_CPIJ009626 Protein, Tsp_08508 Protein, Tsp_12387 Protein, Exoc5 Protein, SEC10 Protein, sec-10 Protein

Hintergrund: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

Molekulargewicht: 82.7 kDa Including tag.

UniProt: O00471

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis