CSTF3 Protein (AA 2-717) (His tag)

Produktnummer ABIN3078497

Produktdetails

Target: CSTF3 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-717

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses CSTF3 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: SGDGATEQAA EYVPEKVKKA EKKLEENPYD LDAWSILIRE AQNQPIDKAR KTYERLVAQF PSSGRFWKLY IEAEIKAKNY DKVEKLFQRC LMKVLHIDLW KCYLSYVRET KGKLPSYKEK MAQAYDFALD KIGMEIMSYQ IWVDYINFLK GVEAVGSYAE NQRITAVRRV YQRGCVNPMI NIEQLWRDYN KYEEGINIHL AKKMIEDRSR DYMNARRVAK EYETVMKGLD RNAPSVPPQN TPQEAQQVDM WKKYIQWEKS NPLRTEDQTL ITKRVMFAYE QCLLVLGHHP DIWYEAAQYL EQSSKLLAEK GDMNNAKLFS DEAANIYERA ISTLLKKNML LYFAYADYEE SRMKYEKVHS IYNRLLAIED IDPTLVYIQY MKFARRAEGI KSGRMIFKKA REDTRTRHHV YVTAALMEYY CSKDKSVAFK IFELGLKKYG DIPEYVLAYI DYLSHLNEDN NTRVLFERVL TSGSLPPEKS GEIWARFLAF ESNIGDLASI LKVEKRRFTA FKEEYEGKET ALLVDRYKFM DLYPCSASEL KALGYKDVSR AKLAAIIPDP VVAPSIVPVL KDEVDRKPEY PKPDTQQMIP FQPRHLAPPG LHPVPGGVFP VPPAAVVLMK LLPPPICFQG PFVQVDELME IFRRCKIPNT VEEAVRIITG GAPELAVEGN GPVESNAVLT KAVKRPNEDS DEDEEKGAVV PPVHDIYRAR QQKRIR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CSTF3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CSTF3 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3))

Andere Bezeichnung: CSTF3 (CSTF3 Produkte)

Synonyme: cstf3 Protein, MGC76035 Protein, CSTF3 Protein, wu:fa99c02 Protein, wu:fb18b09 Protein, zgc:56028 Protein, 4732468G05Rik Protein, C79532 Protein, CstF-77 Protein, cstf3a Protein, CSTF-77 Protein, cleavage stimulation factor, 3' pre-RNA, subunit 3 Protein, cleavage stimulation factor subunit 3 Protein, cstf3 Protein, CSTF3 Protein, LOC100544392 Protein, LOC100641283 Protein, Cstf3 Protein, cstf3.S Protein

Hintergrund: One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs.

Molekulargewicht: 83.7 kDa Including tag.

UniProt: Q12996