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Crk Protein (AA 2-304) (His tag)

Crystallography grade CRK Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Produktnummer ABIN3078398
  • Target Alle Crk (CRK) Proteine anzeigen
    Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-304
    Spezies
    • 6
    • 1
    • 1
    Human
    Quelle
    • 2
    • 2
    • 2
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses Crk Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequenz
    AGNFDSEERS SWYWGRLSRQ EAVALLQGQR HGVFLVRDSS TSPGDYVLSV SENSRVSHYI INSSGPRPPV PPSPAQPPPG VSPSRLRIGD QEFDSLPALL EFYKIHYLDT TTLIEPVSRS RQGSGVILRQ EEAEYVRALF DFNGNDEEDL PFKKGDILRI RDKPEEQWWN AEDSEGKRGM IPVPYVEKYR PASASVSALI GGNQEGSHPQ PLGGPEPGPY AQPSVNTPLP NLQNGPIYAR VIQKRVPNAY DKTALALEVG ELVKVTKINV SGQWEGECNG KRGHFPFTHV RLLDQQNPDE DFS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CRK Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product CRK Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))
    Andere Bezeichnung
    CRK (CRK Produkte)
    Synonyme
    CG1587 Protein, CRK Protein, D-CRK Protein, D-Crk Protein, DCrk Protein, Dcrk Protein, Dmel\\CG1587 Protein, crk Protein, dCRK Protein, dCrk Protein, fb34h10 Protein, fc05a01 Protein, fi19g05 Protein, wu:fb34h10 Protein, wu:fc05a01 Protein, wu:fc54a04 Protein, wu:fi19g05 Protein, zgc:100936 Protein, CRKII Protein, p38 Protein, Crko Protein, crk2 Protein, crkII Protein, v-crk Protein, P38C-CRK Protein, Crk-I Protein, Crk-II Protein, Crk-III Protein, Crk3 Protein, CrkIII Protein, c-Crk Protein, Crk oncogene Protein, v-crk avian sarcoma virus CT10 oncogene homolog Protein, CRK proto-oncogene, adaptor protein Protein, v-crk avian sarcoma virus CT10 oncogene homolog L homeolog Protein, v-crk sarcoma virus CT10 oncogene homolog Protein, Crk Protein, crk Protein, CRK Protein, crk.L Protein
    Hintergrund
    The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. {ECO:0000269|PubMed:11870224, ECO:0000269|PubMed:1630456, ECO:0000269|PubMed:17515907}.
    Molekulargewicht
    34.7 kDa Including tag.
    UniProt
    P46108
    Pathways
    Neurotrophin Signalübertragung, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
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