COPS8 Protein (AA 2-209) (His tag)

Produktnummer ABIN3078267

Produktdetails

Target: COPS8 (COP9 Signalosome Subunit 8 (COPS8))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-209

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses COPS8 Protein ist gelabelt mit His tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Sequenz: PVAVMAESAF SFKKLLDQCE NQELEAPGGI ATPPVYGQLL ALYLLHNDMN NARYLWKRIP PAIKSANSEL GGIWSVGQRI WQRDFPGIYT TINAHQWSET VQPIMEALRD ATRRRAFALV SQAYTSIIAD DFAAFVGLPV EEAVKGILEQ GWQADSTTRM VLPRKPVAGA LDVSFNKFIP LSEPAPVPPI PNEQQLARLT DYVAFLEN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human COPS8 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: COPS8 (COP9 Signalosome Subunit 8 (COPS8))

Andere Bezeichnung: COPS8 (COPS8 Produkte)

Synonyme: COP9 Protein, CSN8 Protein, SGN8 Protein, csn8 Protein, cops8 Protein, MGC76051 Protein, MGC80215 Protein, DDBDRAFT_0167123 Protein, DDBDRAFT_0233107 Protein, DDB_0167123 Protein, DDB_0233107 Protein, zgc:56319 Protein, zgc:86840 Protein, MGC86182 Protein, 9430009J09Rik Protein, AA408242 Protein, Csn8 Protein, Sgn8 Protein, CONSTITUTIVE PHOTOMORPHOGENIC 9 Protein, COP9 SIGNALOSOME SUBUNIT 8 Protein, DL3095C Protein, EMB143 Protein, EMBRYO DEFECTIVE 143 Protein, FCAALL.131 Protein, FUS7 Protein, FUSCA 7 Protein, FUSCA PROTEIN FUS7 Protein, CG13383 Protein, CG33297 Protein, CG42522 Protein, CH8 Protein, Dch8 Protein, Dmel\\CG42522 Protein, Dmel_CG13383 Protein, l(2)SH1829 Protein, l(2)SH2 1829 Protein, COP9 signalosome subunit 8 Protein, COP9 signalosome subunit 8 S homeolog Protein, COP9 signalosome complex subunit 8 Protein, COP9 signalosome subunit 8 L homeolog Protein, COP9 signalosome, subunit CSN8 Protein, COPS8 Protein, Cops8 Protein, cops8 Protein, cops8.S Protein, CSN8 Protein, csn8 Protein, cops8.L Protein, COP9 Protein

Hintergrund: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. {ECO:0000269|PubMed:11285227, ECO:0000269|PubMed:11337588, ECO:0000269|PubMed:12628923, ECO:0000269|PubMed:12732143, ECO:0000269|PubMed:9535219}.

Molekulargewicht: 24.0 kDa Including tag.

UniProt: Q99627

Pathways: Zellzyklus