SSBP3 Protein (AA 1-388) (His tag)
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- Target Alle SSBP3 Proteine anzeigen
- SSBP3 (Single Stranded DNA Binding Protein 3 (SSBP3))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-388
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Spezies
- Human
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses SSBP3 Protein ist gelabelt mit His tag.
- Applikation
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequenz
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MFAKGKGSAV PSDGQAREKL ALYVYEYLLH VGAQKSAQTF LSEIRWEKNI TLGEPPGFLH SWWCVFWDLY CAAPERRDTC EHSSEAKAFH DYSAAAAPSP VLGNIPPNDG MPGGPIPPGF FQGPPGSQPS PHAQPPPHNP SSMMGPHSQP FMSPRYAGGP RPPIRMGNQP PGGVPGTQPL LPNSMDPTRQ QGHPNMGGSM QRMNPPRGMG PMGPGPQNYG SGMRPPPNSL GPAMPGINMG PGAGRPWPNP NSANSIPYSS SSPGTYVGPP GGGGPPGTPI MPSPADSTNS SDNIYTMINP VPPGGSRSNF PMGPGSDGPM GGMGGMEPHH MNGSLGSGDI DGLPKNSPNN ISGISNPPGT PRDDGELGGN FLHSFQNDNY SPSMTMSV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SSBP3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product SSBP3 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- SSBP3 (Single Stranded DNA Binding Protein 3 (SSBP3))
- Andere Bezeichnung
- SSBP3 (SSBP3 Produkte)
- Synonyme
- ssbp3 Protein, MGC75859 Protein, SSBP3 Protein, SSDP1b Protein, si:dkey-63k7.6 Protein, csdp Protein, ssdp Protein, ssdp1 Protein, CSDP Protein, SSDP Protein, SSDP1 Protein, Ssdp Protein, Ssdp3 Protein, SSDP1a Protein, id:ibd1109 Protein, zgc:63791 Protein, 2610021L12Rik Protein, 2610200M23Rik Protein, 5730488C10Rik Protein, AI854733 Protein, AW551939 Protein, LAST Protein, single stranded DNA binding protein 3 Protein, single stranded DNA binding protein 3a Protein, single stranded DNA binding protein 3 L homeolog Protein, single stranded DNA binding protein 3b Protein, single-stranded DNA binding protein 3 Protein, ssbp3 Protein, SSBP3 Protein, ssbp3a Protein, ssbp3.L Protein, Ssbp3 Protein, ssbp3b Protein
- Hintergrund
- May be involved in transcription regulation of the alpha 2(I) collagen gene where it binds to the single-stranded polypyrimidine sequences in the promoter region. {ECO:0000250}.
- Molekulargewicht
- 41.4 kDa Including tag.
- UniProt
- Q9BWW4
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