SLC30A7 Protein (AA 1-376) (Strep Tag)

Produktnummer ABIN3076292

Produktdetails

Target: SLC30A7 (Solute Carrier Family 30 (Zinc Transporter), Member 7 (SLC30A7))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-376

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SLC30A7 Protein ist gelabelt mit Strep Tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequenz: MLPLSIKDDE YKPPKFNLFG KISGWFRSIL SDKTSRNLFF FLCLNLSFAF VELLYGIWSN CLGLISDSFH MFFDSTAILA GLAASVISKW RDNDAFSYGY VRAEVLAGFV NGLFLIFTAF FIFSEGVERA LAPPDVHHER LLLVSILGFV VNLIGIFVFK HGGHGHSHGS GHGHSHSLFN GALDQAHGHV DHCHSHEVKH GAAHSHDHAH GHGHFHSHDG PSLKETTGPS RQILQGVFLH ILADTLGSIG VIASAIMMQN FGLMIADPIC SILIAILIVV SVIPLLRESV GILMQRTPPL LENSLPQCYQ RVQQLQGVYS LQEQHFWTLC SDVYVGTLKL IVAPDADARW ILSQTHNIFT QAGVRQLYVQ IDFAAM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SLC30A7 (Solute Carrier Family 30 (Zinc Transporter), Member 7 (SLC30A7))

Andere Bezeichnung: SLC30A7 (SLC30A7 Produkte)

Synonyme: ZnT-7 Protein, ZnT7 Protein, wu:fi11a08 Protein, zgc:55366 Protein, znt7 Protein, zntl2 Protein, znt-7 Protein, znt7-B Protein, MGC83798 Protein, DKFZp469I1737 Protein, ZNT7 Protein, ZnTL2 Protein, 1810059J10Rik Protein, 2610034N15Rik Protein, 4833428C12Rik Protein, AI467242 Protein, Zntl2 Protein, F3L12.21 Protein, zinc transporter 7 precursor Protein, solute carrier family 30 (zinc transporter), member 7 Protein, solute carrier family 30 (zinc transporter), member 7 L homeolog Protein, solute carrier family 30 member 7 Protein, zinc transporter 7 precursor Protein, slc30a7 Protein, slc30a7.L Protein, SLC30A7 Protein, Slc30a7 Protein, ZIP7 Protein

Hintergrund: Zinc transporter 7 (ZnT-7) (Solute carrier family 30 member 7) (Znt-like transporter 2),FUNCTION: Zinc ion transporter mediating zinc entry from the cytosol into the lumen of organelles along the secretory pathway (PubMed:15525635, PubMed:15994300). By contributing to zinc ion homeostasis within the early secretory pathway, regulates the activation and folding of enzymes like alkaline phosphatases (PubMed:15525635, PubMed:15994300). {ECO:0000269|PubMed:15525635, ECO:0000269|PubMed:15994300}.

Molekulargewicht: 41.6 kDa

UniProt: Q8NEW0

Pathways: Peptide Hormone Metabolism, Transition Metal Ion Homeostasis, SARS-CoV-2 Protein Interaktom