ZNF12 Protein (AA 1-697) (His tag)
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- Target Alle ZNF12 Proteine anzeigen
- ZNF12 (Zinc Finger Protein 12 (ZNF12))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-697
- Spezies
- Human
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses ZNF12 Protein ist gelabelt mit His tag.
- Applikation
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequenz
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MNKSLGPVSF KDVAVDFTQE EWQQLDPEQK ITYRDVMLEN YSNLVSVGYH IIKPDVISKL EQGEEPWIVE GEFLLQSYPD EVWQTDDLIE RIQEEENKPS RQTVFIETLI EERGNVPGKT FDVETNPVPS RKIAYKNSLC DSCEKCLTSV SEYISSDGSY ARMKADECSG CGKSLLHIKL EKTHPGDQAY EFNQNGEPYT LNEESLYQKI RILEKPFEYI ECQKAFQKDT VFVNHMEEKP YKWNGSEIAF LQMSDLTVHQ TSHMEMKPYE CSECGKSFCK KSKFIIHQRT HTGEKPYECN QCGKSFCQKG TLTVHQRTHT GEKPYECNEC GKNFYQKLHL IQHQRTHSGE KPYECSYCGK SFCQKTHLTQ HQRTHSGERP YVCHDCGKTF SQKSALNDHQ KIHTGVKLYK CSECGKCFCR KSTLTTHLRT HTGEKPYECN ECGKFFSRLS YLTVHYRTHS GEKPYECNEC GKTFYLNSAL MRHQRVHTGE KPYECNECGK LFSQLSYLTI HHRTHSGVKP YECSECGKTF YQNSALCRHR RIHKGEKPYE CYICGKFFSQ MSYLTIHHRI HSGEKPYECS ECGKTFCQNS ALNRHQRTHT GEKAYECYEC GKCFSQMSYL TIHHRIHSGE KPFECNECGK AFSRMSYLTV HYRTHSGEKP YECTECGKKF YHKSAFNSHQ RIHRRGNMNV IDVGRLL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ZNF12 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product ZNF12 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- ZNF12 (Zinc Finger Protein 12 (ZNF12))
- Andere Bezeichnung
- ZNF12 (ZNF12 Produkte)
- Synonyme
- 6720465D24 Protein, Krox-6 Protein, Zfp-11 Protein, GIOT-3 Protein, HZF11 Protein, KOX3 Protein, ZNF325 Protein, C530015C18 Protein, Krox-7 Protein, Zfp-12 Protein, Znf12 Protein, zinc finger protein 11 Protein, zinc finger protein 12 Protein, Zfp11 Protein, ZNF12 Protein, Zfp12 Protein
- Hintergrund
- Transcriptional repressor which suppresses activation protein 1 (AP-1)- and serum response element (SRE)-mediated transcriptional activity. {ECO:0000269|PubMed:16806083}.
- Molekulargewicht
- 82.2 kDa Including tag.
- UniProt
- P17014
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