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ZNF367 Protein (AA 1-350) (His tag)

Crystallography grade ZNF367 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, Crys, SDS, WB
Produktnummer ABIN3075718
  • Target Alle ZNF367 Proteine anzeigen
    ZNF367 (Zinc Finger Protein 367 (ZNF367))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-350
    Spezies
    Human
    Quelle
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses ZNF367 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Crystallization (Crys), SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MIRGFEAPMA ENPPPPPPPV IFCHDSPKRV LVSVIRTTPI KPTCGGGGEP EPPPPLIPTS PGFSDFMVYP WRWGENAHNV TLSPGAAGAA ASAALPAAAA AEHSGLRGRG APPPAASASA AASGGEDEEE ASSPDSGHLK DGIRRGRPRA DTVRDLINEG EHSSSRIRCN ICNRVFPREK SLQAHKRTHT GERPYLCDYP DCGKAFVQSG QLKTHQRLHT GEKPFVCSEN GCLSRFTHAN RHCPKHPYAR LKREEPTDTL SKHQAADNKA AAEWLARYWE MREQRTPTLK GKLVQKADQE QQDPLEYLQS DEEDDEKRGA QRRLQEQRER LHGALALIEL ANLTGAPLRQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ZNF367 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    ZNF367 (Zinc Finger Protein 367 (ZNF367))
    Andere Bezeichnung
    ZNF367 (ZNF367 Produkte)
    Synonyme
    MGC81504 Protein, AFF29 Protein, CDC14B Protein, ZFF29 Protein, 8030486J21Rik Protein, ZFF29a Protein, ZFF29b Protein, Znf367 Protein, hydroxysteroid 17-beta dehydrogenase 3 Protein, zinc finger protein 367 S homeolog Protein, zinc finger protein 367 Protein, zinc finger 367-like protein Protein, HSD17B3 Protein, znf367.S Protein, ZNF367 Protein, Zfp367 Protein
    Hintergrund
    Transcriptional activator. Isoform 1 may be involved in transcriptional activation of erythroid genes. {ECO:0000269|PubMed:15344908}.
    Molekulargewicht
    39.4 kDa Including tag.
    UniProt
    Q7RTV3
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