VPS28 Protein (AA 1-221) (Strep Tag)

Produktnummer ABIN3075431

Produktdetails

Target: VPS28 (Vacuolar Protein Sorting 28 (VPS28))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-221

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses VPS28 Protein ist gelabelt mit Strep Tag.

Applikation: SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Sequenz: MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: VPS28 (Vacuolar Protein Sorting 28 (VPS28))

Andere Bezeichnung: VPS28 (VPS28 Produkte)

Synonyme: CG12770 Protein, DmVps28 Protein, Dmel\\CG12770 Protein, Dvps28 Protein, ESCRT-I Protein, ESCRT-II Protein, dVps28 Protein, dvps28 Protein, l(2)k16503 Protein, vps28 Protein, VPS28 Protein, GB12925 Protein, VPL13 Protein, VPT28 Protein, DDBDRAFT_0186436 Protein, DDBDRAFT_0234023 Protein, DDB_0186436 Protein, DDB_0234023 Protein, 1110014J03Rik Protein, AI847241 Protein, D730005C08Rik Protein, zgc:66078 Protein, Vacuolar protein sorting 28 Protein, VPS28, ESCRT-I subunit Protein, vacuolar protein sorting 28 homolog Protein, vacuolar protein sorting-associated protein 28 homolog Protein, ESCRT-I subunit protein VPS28 Protein, ESCRT I complex subunit Vps28 Protein, transport of soluble vacuolar hydrolase precursors Protein, subunit of the ESCRT-I complex Protein, vacuolar protein sorting-associated protein Protein, vacuolar protein sorting 28 family protein Protein, vacuolar protein sorting-associated protein 28 Protein, Vacuolar protein sorting-associated protein 28 homolog Protein, vacuolar protein sorting 28 Protein, vacuolar protein sorting 28 (yeast) Protein, vacuolar protein sorting 28 homolog S homeolog Protein, Vps28 Protein, VPS28 Protein, vps28 Protein, LOC408783 Protein, CpipJ_CPIJ003920 Protein, LACBIDRAFT_399656 Protein, PTRG_04781 Protein, MCYG_00028 Protein, PITG_04534 Protein, Tsp_08691 Protein, Tsp_08687 Protein, Tsp_08688 Protein, vps-28 Protein, vps28.S Protein

Hintergrund: Vacuolar protein sorting-associated protein 28 homolog (H-Vps28) (ESCRT-I complex subunit VPS28),FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. {ECO:0000269|PubMed:11916981}.

Molekulargewicht: 25.4 kDa

UniProt: Q9UK41