UCK2 Protein (AA 2-261) (His tag)

Produktnummer ABIN3075182

Produktdetails

Target: UCK2 (Uridine-Cytidine Kinase 2 (UCK2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-261

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses UCK2 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: AGDSEQTLQN HQQPNGGEPF LIGVSGGTAS GKSSVCAKIV QLLGQNEVDY RQKQVVILSQ DSFYRVLTSE QKAKALKGQF NFDHPDAFDN ELILKTLKEI TEGKTVQIPV YDFVSHSRKE ETVTVYPADV VLFEGILAFY SQEVRDLFQM KLFVDTDADT RLSRRVLRDI SERGRDLEQI LSQYITFVKP AFEEFCLPTK KYADVIIPRG ADNLVAINLI VQHIQDILNG GPSKRQTNGC LNGYTPSRKR QASESSSRPH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human UCK2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: UCK2 (Uridine-Cytidine Kinase 2 (UCK2))

Andere Bezeichnung: UCK2 (UCK2 Produkte)

Synonyme: TSA903 Protein, UK Protein, UMPK Protein, AA407809 Protein, AI481316 Protein, AU018180 Protein, AU020720 Protein, UMK Protein, Umpk Protein, wu:fa20g04 Protein, zgc:56174 Protein, UCK2 Protein, DDBDRAFT_0202355 Protein, DDBDRAFT_0216233 Protein, DDB_0202355 Protein, DDB_0216233 Protein, DDBDRAFT_0167798 Protein, DDBDRAFT_0230208 Protein, DDB_0167798 Protein, DDB_0230208 Protein, UCK 2-A Protein, umpk Protein, wu:fk91a01 Protein, zgc:66240 Protein, uridine-cytidine kinase 2 Protein, uridine-cytidine kinase 2b Protein, microRNA 3658 Protein, uridine-cytidine kinase 2 S homeolog Protein, uridine kinase Protein, Uridine kinase Protein, toxin secretion ABC transporter ATP-binding protein Protein, uridine-cytidine kinase 2a Protein, UCK2 Protein, Uck2 Protein, uck2b Protein, MIR3658 Protein, uck2.S Protein, LOC692983 Protein, PTRG_08949 Protein, udkA Protein, udkB Protein, Mrub_2364 Protein, Mesil_2200 Protein, Spirs_2239 Protein, Tmar_0146 Protein, Bache_3005 Protein, Celal_2781 Protein, Deima_0461 Protein, Odosp_0439 Protein, Bacsa_1376 Protein, Celly_0243 Protein, Weevi_1813 Protein, Marky_0973 Protein, Spico_0967 Protein, Poras_0295 Protein, Theth_1915 Protein, uck2 Protein, XCC1480 Protein, uck2a Protein

Hintergrund: Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.

Molekulargewicht: 30.1 kDa Including tag.

UniProt: Q9BZX2

Pathways: Ribonucleoside Biosynthetic Process, Feeding Behaviour