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BRF1 Protein (AA 1-677) (His tag)

Crystallography grade BRF1 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3074152
  • Target Alle BRF1 Proteine anzeigen
    BRF1 (BRF1, RNA polymerase III transcription initiation factor subunit (BRF1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-677
    Spezies
    • 2
    • 1
    • 1
    Human
    Quelle
    • 1
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses BRF1 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MTGRVCRGCG GTDIELDAAR GDAVCTACGS VLEDNIIVSE VQFVESSGGG SSAVGQFVSL DGAGKTPTLG GGFHVNLGKE SRAQTLQNGR RHIHHLGNQL QLNQHCLDTA FNFFKMAVSR HLTRGRKMAH VIAACLYLVC RTEGTPHMLL DLSDLLQVNV YVLGKTFLLL ARELCINAPA IDPCLYIPRF AHLLEFGEKN HEVSMTALRL LQRMKRDWMH TGRRPSGLCG AALLVAARMH DFRRTVKEVI SVVKVCESTL RKRLTEFEDT PTSQLTIDEF MKIDLEEECD PPSYTAGQRK LRMKQLEQVL SKKLEEVEGE ISSYQDAIEI ELENSRPKAK GGLASLAKDG STEDTASSLC GEEDTEDEEL EAAASHLNKD LYRELLGGAP GSSEAAGSPE WGGRPPALGS LLDPLPTAAS LGISDSIREC ISSQSSDPKD ASGDGELDLS GIDDLEIDRY ILNESEARVK AELWMRENAE YLREQREKEA RIAKEKELGI YKEHKPKKSC KRREPIQAST AREAIEKMLE QKKISSKINY SVLRGLSSAG GGSPHREDAQ PEHSASARKL SRRRTPASRS GADPVTSVGK RLRPLVSTQP AKKVATGEAL LPSSPTLGAE PARPQAVLVE SGPVSYHADE EADEEEPDEE DGEPCVSALQ MMGSNDYGCD GDEDDGY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human BRF1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product BRF1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    BRF1 (BRF1, RNA polymerase III transcription initiation factor subunit (BRF1))
    Andere Bezeichnung
    BRF1 (BRF1 Produkte)
    Synonyme
    BRF Protein, BRF-1 Protein, GTF3B Protein, TAF3B2 Protein, TAF3C Protein, TAFIII90 Protein, TF3B90 Protein, TFIIIB90 Protein, hBRF Protein, DDBDRAFT_0189159 Protein, DDBDRAFT_0216283 Protein, DDB_0189159 Protein, DDB_0216283 Protein, 2510002F24Rik Protein, mTFIIIB90 Protein, BRF1 Protein, ZFP36L1 Protein, CG31256 Protein, CG4155 Protein, CG5419 Protein, Dmel\\CG31256 Protein, TFIIIB Protein, brf Protein, brf1 Protein, wu:fi47f03 Protein, zgc:55543 Protein, fi40c10 Protein, wu:fi40c10 Protein, zgc:63497 Protein, BRF1, RNA polymerase III transcription initiation factor subunit Protein, transcription initiation factor IIB Protein, general transcription factor IIB Protein, cilia and flagella associated protein 20 Protein, transcription factor IIb, putative Protein, transcription factor IIB Protein, transcription factor IIb Protein, Transcription factor iib, putative Protein, BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit Protein, CG31256 gene product from transcript CG31256-RA Protein, BRF1, RNA polymerase III transcription initiation factor a Protein, BRF1, RNA polymerase III transcription initiation factor b Protein, BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit L homeolog Protein, BRF1 Protein, gtf2b Protein, GTF2B Protein, cfap20 Protein, PFE0415w Protein, Tb09.160.4220 Protein, PVX_097980 Protein, CpipJ_CPIJ011738 Protein, CpipJ_CPIJ014799 Protein, CpipJ_CPIJ018710 Protein, PKH_102450 Protein, Brf1 Protein, Brf Protein, brf1a Protein, brf1b Protein, brf1.L Protein
    Hintergrund
    General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
    Molekulargewicht
    74.8 kDa Including tag.
    UniProt
    Q92994
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