PDCD1LG2 Protein (AA 20-219) (His tag,AVI tag,Biotin)

Produktnummer ABIN2870560

Produktdetails

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 20-219

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PDCD1LG2 Protein ist gelabelt mit His tag,AVI tag,Biotin.

Marke: MABSol®,PrecisionAvi

Sequenz: AA 20-219

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Produktmerkmale: This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 25.2 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen

Tocheva, Lerrer, Mor: "In Vitro Assays to Study PD-1 Biology in Human T Cells." in: Current protocols in immunology, Vol. 130, Issue 1, pp. e103, (2020) (PubMed).

Arlauckas, Garris, Kohler, Kitaoka, Cuccarese, Yang, Miller, Carlson, Freeman, Anthony, Weissleder, Pittet: "In vivo imaging reveals a tumor-associated macrophage-mediated resistance pathway in anti-PD-1 therapy." in: Science translational medicine, Vol. 9, Issue 389, (2018) (PubMed).

Antigendetails

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Andere Bezeichnung: PD-L2 (PDCD1LG2 Produkte)

Synonyme: PDL2 Protein, B7DC Protein, Btdc Protein, CD273 Protein, PD-L2 Protein, PDCD1L2 Protein, bA574F11.2 Protein, B7-DC Protein, F730015O22Rik Protein, programmed cell death 1 ligand 2 Protein, PDCD1LG2 Protein, Pdcd1lg2 Protein

Hintergrund: Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

Molekulargewicht: 25.2 kDa